Aβ1-42猴β淀粉样蛋白1-42酶联免疫试剂盒操作步骤：

1. 编号：将样品对应微孔按序编号，每板应设阴性对照 2 孔、阳性对照 2 孔、空白对照 1孔（空白对照孔不加样品及酶标试剂，其余各步操作相同）

2. 加样：分别在阴、阳性对照孔中加入阴性对照、阳性对照 50%l。然后在待测样品孔先加样品稀释液 40%l，然后再加待测样品 10%l。加样将样品加 于酶标板孔底部，尽量不触及孔壁，轻轻晃动混匀，

3. 温育：用封板膜封板后置 37℃温育 30 分钟。

4. 配液：将 30 倍浓缩洗涤液用蒸馏水 30 倍稀释后备用

5. 洗涤：小心揭掉封板膜，弃去液体，甩干，每孔加满洗涤液，静置 30 秒后弃去，如此重复 5 次，拍干。

6. 加酶：每孔加入酶标试剂 50%l，空白孔除外。

7. 温育：操作同 3。

8. 洗涤：操作同 5。

9. 显色：每孔先加入显色剂 A50%l，再加入显色剂 B50%l，轻轻震荡混匀，37℃避光显色15 分钟.

10. 终止：每孔加终止液 50%l，终止反应（此时蓝色立转黄色）。

11. 测定：以空白空调零，450nm 波长依序测量各孔的吸光度（OD 值）。 测定应在加终止液后 15 分钟以内进行。

兔抗羊IgG (抗血清) Anti-Goat IgG antibody produced in rab... 1ML 免疫血清

兔抗豚鼠IgG (抗血清) Anti-Guinea Pig IgG antibody produced ... 1ML 免疫血清

羊抗马IgG (抗血清) Anti-Horse IgG antibody produced in goat 1ML 免疫血清

兔抗马IgG (抗血清) Anti-Horse IgG antibody produced in ra... 1ML 免疫血清

羊抗人白蛋白 (抗血清) Anti-Human Albumin antibody produced i... 1ML 免疫血清

兔抗人白蛋白 (抗血清) Anti-Human Albumin antibody produced i... 1ML 免疫血清

羊抗人C3 (抗血清) Anti-Human C3 antibody produced in goat 1ML 免疫血清

羊抗人纤维蛋白原 (抗血清) Anti-Human Fibrinogen produced in goat 1ML 免疫血清

兔抗人纤维蛋白原 (抗血清) Anti-Human Fibrinogen produced in rabbit 1ML 免疫血清

羊抗人IgA血清 (α链特异) Anti-Human IgA (α-chain spec.) produc... 1ML 免疫血清

羊抗人IgA (抗血清) Anti-Human IgA antibody produced in goat 1ML 免疫血清

兔抗人IgA (抗血清) Anti-Human IgA antibody produced in ra... 1ML 免疫血清

兔抗人IgG～Fc (抗血清) Anti-Human IgG (Fc spec.) produced in ... 1ML 免疫血清

羊抗人IgG (抗血清) Anti-Human IgG antibody produced in goat 1ML 免疫血清

兔抗人IgG (抗血清) Anti-Human IgG antibody produced in ra... 1ML 免疫血清

羊抗人IgM血清 (μ链特异) Anti-Human IgM (μ-chain spec.) produc... 1ML 免疫血清

羊抗人IgM (抗血清) Anti-Human IgM antibody produced in goat 1ML 免疫血清

兔抗人IgM (抗血清) Anti-Human IgM antibody produced in ra... 1ML 免疫血清

羊抗人血清 (多价抗体) Anti-Human Serum antibody produced in ... 1ML 免疫血清

兔抗人血清 (多价抗体) Anti-Human Serum antibody produced in ... 1ML 免疫血清

兔抗猴IgG (抗血清) Anti-Monkey IgG antibody produced in r... 1ML 免疫血清

兔抗小鼠IgG～Fc (抗血清) Anti-Mouse IgG (Fc spec.) produced in ... 1ML 免疫血清

羊抗小鼠IgG (抗血清) Anti-Mouse IgG antibody produced in goat 1ML 免疫血清

兔抗小鼠IgG (抗血清) Anti-Mouse IgG antibody produced in ra... 1ML 免疫血清

兔抗小鼠血清 (多价抗体) Anti-Mouse Serum antibody produced in ... 1ML 免疫血清

兔抗HRP (抗血清) Anti-Peroxidase antibody produced in r... 1ML 免疫血清

羊抗猪IgG (抗血清) Anti-Pig IgG antibody produced in goat 1ML 免疫血清

羊抗兔IgG (抗血清) Anti-Rabbit IgG antibody produced in g... 1ML 免疫血清

羊抗大鼠IgG (抗血清) Anti-Rat IgG antibody produced in goat 1ML 免疫血清