ICG maleimide,2143933-81-5，吲哚菁绿

英文名：ICG maleimide，ICG-MAL

中文名：吲哚菁绿-马来酰亚胺

外观：绿色粉末

溶解度：二氯甲烷

纯度：95%+

结构式：

ICG maleimide,2143933-81-5，吲哚菁绿

ICG is a tricarbocyanine-type dye with NIR-absorbing properties (peak absorption around 800 nm) and emission maximum at 807 nm (10% v/vDMSO/PBS). Little absorption in the visible range accounts for the low autofluorescence, tissue absorbance, and scattering at NIR wavelengths (700-900 nm).

The maleimide of the ICG dye offers the opportunity to develop optimal conjugates. Maleimide active group provides an efficient and convenient way to selectively link ICG dye to sulfhydryl groups (free thiol, R-SH) on various substrates (antibodies[1], peptides, proteins, oligonucleotides, small molecule drugs etc.) at neutral (physiological) pH without any activation. Maleimides have very low reactivity with amines, alcohols, and phenols (incl. tyrosine and histidine) and do not react with histidine and methionine thus providing very high labeling selectivity.

It has been reported that conjugation to some antibodies may cause decrease in fluorescence intensity of ICG bound to antibody due to the formation of H-dimers4. However, fluorescence is restored after cell binding and internalization, which can be used to design an activatable NIR fluorescent probe. Fluorescence intensity of the unbound ICG-antibody conjugate can be dramatically increased (up to 50-fold) by addition of SDS and β-mercaptoethanol, which diminishes π- π stacking5.

近红外荧光染料Near IRDyes激发和发射波长和颜色图：

ICG NHS ester的NHS可以和蛋白、多肽或抗体上面的NH2发生反应链接到一起。

ICG-maleimide的马来酰亚胺可以和蛋白、多肽、小分子或抗体上的SH巯基反应连接。

ICG-Amine的NH2可以和蛋白、多肽小分子上面的NHS反应连接起来。

ICG和PEG链接可在体内长循环。

  
ICG衍生物产品：
ICG修饰蛋白类产品
ICG-Avidin
ICG-Streptavidin
ICG-BSA      
ICG-Dextran
ICG-Alginate
ICG-Chitosan
ICG-Hyaluronic acid
ICG-Heparin
ICG-Ficoll
ICG-Cellulose
ICG-Concanavalin A
ICG-Transferrin
ICG-Polylysine
ICG NHS ester
ICG Vinylsulfone
ICG isothiocyanate
ICG Carboxlaic acid
ICG Maleimide
ICG amine
ICG thiol
ICG dichlorotriazine
ICG Hydrazide
ICG alkyne
ICG Avidin
ICG Biotin

吲哚菁绿直接标记在磷脂上:
DSPE-ICG
DPPE-ICG
DMPE-ICG
DLPE-ICG
DOPE-ICG
 
吲哚菁绿通过一个小分子PEG偶连到磷脂上,PEG重复单元从2-5个不等:
DSPE-PEG2-ICG
DSPE-PEG3-ICG
DSPE-PEG4-ICG
 
吲哚菁绿连接高分子PEG再连接到磷脂上，PEG分子量有550/750/1000/2000/3400/5000
ICG-PEG-DSPE
ICG-PEG-DMPE
ICG-PEG-DPPE
ICG-PEG-DLPE
ICG-PEG-DOPE
 
吲哚菁绿标记PEG末端带活性基团产品,PEG分子量1000/2000/3400/5000 Da ：
mPEG-ICG
ICG-PEG-NH2
ICG-PEG-COOH
ICG-PEG-MAL
ICG-PEG-NHS
ICG-PEG-N3
ICG-PEG-Biotin
ICG-PEG-SH
ICG-PEG-OH
ICG-PEG-Alkyne
ICG-PEG-CHO
ICG-PEG-Folate
ICG-PEG-cRGD
 
吲哚菁绿修饰一些聚合物产品：
PLGA-ICG
PCL-ICG
PDLLA-ICG
Dextran-ICG
PEI-ICG
PAMAM-ICG
PLL-ICG
 
吲哚菁绿修饰一些共聚物产品：
PLGA-PEG-ICG
PCL-PEG-ICG
PLA-PEG-ICG
mPEG-PLGA-ICG
mPEG-PLA-ICG
mPEG-PCL-ICG
PLGA-PEI-ICG
Dextran-PLGA-ICG
Dextran-PCL-ICG
ICG-PEG-PEI