Cy5酰肼Cyanine5 hydrazide用于标记醛和酮的活性染料-技术文章-上海惠诚生物科技有限公司手机版

Cy5酰肼Cyanine5 hydrazide用于标记醛和酮的活性染料

时间：2020-06-30 阅读：604

Cyanine5 hydrazide是用于标记醛和酮的活性染料。是Cy5 hydrazide的类似物。
该染料能够很容易与含羰基的分子以近乎理想化的程度反应。比如承受氧化压力的蛋白，糖基化的蛋白通过高碘酸氧化作用重新活化（包括抗体），含醛基的寡核苷酸。
Cyanine5 hydrazide可以替代carbonyl-reactive Cy5, Alexa Fluor 647, DyLight 649等染料。

Chemical Name

Cy5 酰肼 Cyanine5 hydrazide

Mol. Formula

C32H42Cl2N4O

Mol. Weight

569.61

Qty 1

5mg

Qty 2

25mg

Appearance

dark blue powder

Solubility

moderate solubility in water, good in polar organic solvents (DMF, DMSO, alcohols)

Storage condition

-20°C

References

Cyanine5 hydrazide is a reactive dye for the labeling of aldehydes and ketones, an analog of Cy5® hydrazide.

This dye reacts smoothly and nearly quantitatively with various carbonyl groups encountered in biomolecules. Examples are proteins subjected to oxidative stress, glycosylated proteins pre-activated by periodate oxidation (including antobodies), and oligonucleotides with aldehyde moieties.

Cyanine5 hydrazide replaces carbonyl-reactive Cy5®, Alexa Fluor 647, DyLight 649 dyes.

Spectral properties

Excitation maximum, nm:	646
ε, L⋅mol−1⋅cm−1:	250000
Emission maximum, nm:	662
Fluorescence quantum yield:	0.2

Product citations

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Huang, J.; Smith, F.; Panizzi, J.R.; Goodwin, D.C.; Panizzi, P. Inactivation of myeloperoxidase by benzoic acid hydrazide. Archives of Biochemistry and Biophysics, 2015, 570, 14–22. doi:10.1016/j.abb.2015.01.028
Einfalt, T.; Goers, R.; Dinu, I.A.; Najer, A.; Spulber, M.; Onaca-Fischer, O.; Palivan, C.G. Stimuli-triggered activity of nanoreactors by biomimetic engineering polymer membranes. Nano Letters, 2015, 15(11), 7596–7603. doi: 10.1021/acs.nanolett.5b03386