Biomomentum多轴机械测试仪Mach-1应用：

Automated Indentation Demonstrates Structural Stiffness of Femoral Articular Cartilage and Temporomandibular Joint Mandibular Condylar Cartilage Is Altered in FgF2KO Mice

Paige S. Woods, Alyssa A. Morin, Po-Jung Chen, Sarah Mahonski, Liping Xiao, Marja Hurley, Sumit Yadav, and Tannin A. Schmidt

CARTILAGE. October 2020. doi:10.1177/1947603520962565

Objective

Employ an automated indentation technique, using a commercially available machine, to assess the effect of fibroblast growth factor 2 (FGF2) expression on structural stiffness over the surface of both murine femoral articular cartilage (AC) and temporomandibular joint (TMJ) mandibular condylar cartilage (MCC).

Design

Experiments were performed using 3-month-old female homozygote Fgf2KO mice with wild type (WT) littermates. After euthanization, isolated mandibles and hindlimbs were either processed for histology or subjected to automated indentation on a Biomomentum Mach-1 v500csst with a 3-axis motion controller in a phosphate buffered saline bath using a 0.3 mm spherical tip indenter. The effect of indentation depth on normal force was characterized, then structural stiffness was calculated and mapped at multiple positions on the AC and MCC.

Results

Automated indentation of the AC and TMJ MCC was successfully completed and was able to demonstrate both regional variation in structural stiffness and differences between WT and Fgf2KO mice. Structural stiffness values for Fgf2KO AC were significantly smaller than WT at both the medial/anterior (P < 0.05) and medial/posterior (P < 0.05) positions. Global Fgf2KO also lead to a decrease in MCC thickness of the TMJ compared with WT (P < 0.05) and increased structural stiffness values for Fgf2KO at both the posterior and anterior location (P < 0.05).

Conclusions

Biomomentum多轴机械测试仪Mach-1应用：自动压痕表明FgF2KO小鼠股骨软骨的结构刚度和颞下颌关节下颌Con突软骨发生改变。

采用自动压痕技术，使用市售机器评估成纤维细胞生长因子2（FGF2）表达对鼠股骨关节软骨（AC）和颞下颌关节（TMJ）下颌con突软骨表面的结构刚度的影响（我的客户中心（MCC）。设计使用具有野生型（WT）同窝仔的3个月大的雌性纯合子Fgf2KO小鼠进行实验。对分离出的下颌骨和后肢进行组织学处理，或在带有3-轴运动控制器的Biomomentum Mach-1 v500csst上使用0.3毫米球形尖压头在磷酸盐缓冲盐水浴中进行自动压痕。表征了压痕深度对法向力的影响，然后计算结构刚度并将其映射到AC和MCC的多个位置。结果AC和TMJ MCC的自动压痕已成功完成，并且能够证明结构刚度的区域差异以及WT和Fgf2KO小鼠之间的差异。Fgf2KO AC的结构刚度值在内侧/前侧（P <0.05）和内侧/后侧（P <0.05）位置均显着小于WT。与WT相比，整体Fgf2KO还会导致TMJ的MCC厚度减小（P <0.05），并且在后部和前部位置的Fgf2KO的结构刚度值都会增加（P <0.05）。