昊然生物-Alexa Fluor594-streptavidin,荧光AF594可以标记蛋白/抗体
时间:2023-09-26 阅读:236
昊然生物-Alexa Fluor594-streptavidin,荧光AF594可以标记蛋白/抗体
昊然生物分享荧光AF594可以标记蛋白/抗体:
文献描述:
Fluorescent dye alexa-fluor-594 was fixed to biotin with a spacer chain of four atoms. The static, dynamic fluorescence and fluorescence polarization properties of the avidin/labeled-biotin conjugate were investigated and compared with those of free fluorescent dye and directly labeled avidin. With 0.8 label on the average per avidin, one single fluorescence lifetime and one single correlation time related to the actual protein motion were recorded. Moreover, the excited state fluorescent probe is stabilized in the conjugate, with a quantum yield of 0.87, by slowing down the non-radiative processes, while fluorescence lifetime and fluorescence quantum yield increase by 10%, with respect to free alexa-fluor-594.
产品名称:Alexa Fluor594-streptavidin
中文名称:AF594标记链霉亲和素
英文名称:Alexa Fluor594-streptavidin
分类:蛋白标记
溶解性:/
规格:mg
纯度:95%+
储存:/
用途:科研
供货商:西安昊然生物科技有限公司
Alexa Fluor 594可以通过生物SU-链霉亲和素(Biotin-Streptavidin)相互作用来偶联链霉亲和素。这种方法通常用于将Alexa Fluor 594或其他荧光染料标记到生物su修饰的分子或颗粒上,以进行生物标记、荧光显微镜成像或流式细胞仪分析等应用。
可以使用激发适当波长的光来激发Alexa Fluor 594或其他荧光染料,然后检测并记录荧光信号。这可以通过荧光显微镜、流式细胞仪或荧光分光光度计等设备来完成。
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西安昊然仅用于科研,RL2023.9