Cedarlane 淋巴细胞分离柱-相关技术文章
时间：2024-07-16 阅读：89
Cedarlane 淋巴细胞分离柱
Cedarlane公司成立于加拿大，27年间，已经逐步发展成为生命科学行业的一家跨国公司，在加拿大和美国拥有超过100名员工。
Cedarlane公司的研究领域。
公司为生命科学研究人员和临床医生提供无数的生物和生化产品，提供来自几乎所有世jie上zui 知 ming 的国际制造商的产品。交货及时，所有产品都在适当的所需温度下储存、接收和运输。员工与客户和供应商密切合作，提供个性化和全面的体验，以反映客户至上的核心价值。通过提供全球超过 600 万种试剂盒和试剂的门户，客户拥有货运整合的优势以及其中固有的便利性和成本节约;Cedarlane 是一站式商店。
主要分销产品线包括：抗体、细胞培养基和过滤、原代细胞和细胞系、蛋白质和肽、酶和抑制剂、成像和显微镜资源、检测/ELISA试剂盒、精细化学品和生化、DNA/RNA分离和纯化。
主要制造产品包括：单克隆和多克隆抗体、细胞分离培养基、组织分型补体和高度表征的神经元细胞系。
Lympholyte®-H是一种密度梯度分离介质，专门设计用于从人外周血和脐带血中分离淋巴细胞和单核细胞。
注意：使用前充分摇晃混匀，静止2-3min，待气泡消失后试用。（室温条件下使用分离液，分离液密度会随温度的变化而发生改变）
样本制备可使用无血清培养基，减少样本粘度，更利于分离。
操作步骤：
1.在含有抗凝血剂的试管中收集人血；
2.用等量培养基1：1稀释血液；
3.根据不同的方法进行分离：
①在离心管中加入3 mL Lympholyte®-H；
②使用移液管，小心地将6 mL稀释的血液铺在Lympholyte®-H上，在液面上尽可能少地混合（图A）；
③由于Lympholyte®-H的密度比细胞悬液更大，因此将形成一个明显的界面（图C）。
方法B：
①在离心管中加入6 mL稀释后的血液。将一个大的(23厘米）移液管放置到试管的底部（图B）；
②慢慢地向移液管中加入Lympholyte®-H，重力使其在稀释的血液下分层；
③继续处理，直到3mL Lympholyte®-H在稀释的血液下分层。由于Lympholyte®-H比细胞悬液密度更大，因此会形成明显的界面（图C）；
4.在室温下，以800g离心20分钟；
5.离心后，界面上将有一个明显的淋巴细胞层（图D）。使用移液管，小心地将细胞吸出，并转移到一个新的离心管中；
6.用培养基稀释转移的细胞，以降低溶液的密度。800g离心10分钟，沉淀淋巴细胞，然后弃用上清液；
Note：如果需要去除血小板，可在这一步进行操作：
方法A
A.加入0.5 mL缓冲液和50 uL凝血酶，然后轻轻重悬细胞；
B.用缓冲液补足10mL，并充分混合；
C.非常缓慢地(100g）离心细胞3分钟；血小板会聚集并沉淀在底部；
D. 取出上清液（包含细胞），放入另一个试管中，留下聚集的血小板。
方法B
A.800 g离心1分钟，沉淀细胞；
B.倒出上清液，用缓冲液重悬细胞，重复2次；
C.此时会观察到，当血小板被移除时，产生的上清液会变得更加清晰。
7.在进一步处理之前，在培养基中清洗淋巴细胞2-3次（在这个阶段可以使用含有血清的培养基）。
以下是Cedarlane公司的热销产品
产品名称 | 货号 | 规格 |
Lympholyte®-H Cell Separation Media (Isolation of lymphocytes from Human peripheral blood), sterile liquid | CL5020 | 500ml |
Baby Rabbit Complement, Tissue Culture Grade - Frozen | CL3441-S | 1ml |
Lympholyte®-H Cell Separation Media (Isolation of lymphocytes from Human peripheral blood), sterile liquid | CL5016 | 6x100mL |
Low-Tox® Guinea Pig Complement, Lyophilized | CL4051 | 5x1ml |
Lympholyte®-M Cell Separation Media (Isolation of lymphocytes from Mouse lymphoid tissue), sterile liquid | CL5031 | 100ml |
Lympholyte®-Mammal Cell Separation Media (Isolation of lymphocytes from Mammalian peripheral blood), sterile liquid | CL5115 | 100ml |
Low-Tox®-M Rabbit Complement, Lyophilized | CL3051 | 5x1ml |
Lympholyte®-M Cell Separation Media (Isolation of lymphocytes from Mouse lymphoid tissue), sterile liquid | CL5030 | 5x30ml |
Lympholyte®-H Cell Separation Media (Isolation of lymphocytes from Human peripheral blood), sterile liquid | CL5015 | 100ml |
Lympholyte®-H Cell Separation Media (Isolation of lymphocytes from Human peripheral blood), sterile liquid | CL5026 | 6x500ml |
Anti-Mouse Asialo GM1, Ig Fraction (Polyclonal)(rabbit Ig) | CL8955 | 1 mL |
Lympholyte®-Mammal Cell Separation Media (Isolation of lymphocytes from Mammalian peripheral blood), sterile liquid | CL5120 | 500ml |
Human Complement, Pooled, Frozen | CL6600-1 | 1 ml |
Anti-Mouse/Human Mac-2 (Galectin-3), Purified (Clone M3/38) (rat IgG2a) | CL8942AP | 100ug |
Anti-Human Tamm Horsfall Purifed (Clone 10.32) (mouse IgG2b) | CL1032AP | 250ug |
Anti-Mouse Complement Component C3, FITC (Clone RmC11H9) (rat IgG2a) | CL7503F | 100ug |
Lympholyte®-Mammal Cell Separation Media (Isolation of lymphocytes from Mammalian peripheral blood), sterile liquid | CL5110 | 5x30ml |
Lympholyte®-1.1, sterile liquid | CL6155 | 500mL |
Baby Rabbit Complement, Lyophilized | CL3441 | 10x1ml |
Anti-Human Factor VIII:C HRPO (Polyclonal) (sheep IgG) | CL20035HP | 0.2 mg |
Anti-Human Factor VIII:C Aff.Pur.HRPO(Paired Abs) | CL20035K | 4 plates |
Low-Tox®-H Rabbit Complement, Lyophilized | CL3331 | 10x1mL |
Chicken Gamma Globulin (purified antigen) | CLF802-1 | 50mg |
Lympholyte®-Rat Cell Separation Media (Isolation of lymphocytes from Rat lymphoid tissue), sterile liquid | CL5041 | 100mL |
Lympholyte®-Rabbit Cell Separation Media (Isolation of lymphocytes from Rabbit lymphoid tissue), sterile liquid | CL5050 | 5x30ml |
Standard Rabbit Complement, Lyophilized | CL3111 | 25x1ml |
Anti-Rat CD90 (Thy 1.1) Ascites (Clone OX-7) (mouse IgG1) | CL005A | 0.5ml |
Rabbit Complement MA, Lyophilized | CL3221 | 10x1ml |
Lympholyte®-poly Cell Separation Media (Isolation of PMNs from Human peripheral blood), sterile liquid | CL5070 | 100mL |
Anti-Human Fibrinogen Aff.Pur./HRPO (Paired Abs) | CL20067K | 5 plates |
Lympholyte®-1.1, sterile liquid | CL5095 | 500mL |
O-sialoglycoprotein endopeptidase, Lyophilized | CLE100 | 1.2mg |
Anti-Mouse Thrombocyte (platelet), Antiserum, (Polyclonal) (rabbit serum) | CLA31440 | 5mL |
Anti-Human Tissue Plasminogen Activator, Affinity Purified (Polyclonal) (sheep IgG) | CL20115AP | 0.5 mg |
Anti-Human Factor IX Aff.Pur./HRPO (Paired Abs) | CL20041K | 5 plates |
Baby Rabbit Complement, Tissue Culture Grade, Frozen | CL3441-S100 | 100ml |
Standard Guinea Pig Complement, Lyophilized | CL5000 | 5x5ml |
Lympholyte®-H Cell Separation Media (Isolation of lymphocytes from Human peripheral blood), sterile liquid | CL5010 | 5x30mL |
Human Interleukin-17A | CL101-17-25UG | 25ug |
Anti-Mouse Collagen Type I, purified IgG | CL50151AP-1 | 0.1ml |
Anti-Mouse CD25 , Purified (Clone PC61.5.3) (rat IgG1) | CL8925AP | 250ug |
Anti-Rat IL-10, Purified, (Polyclonal) (rabbit IgG) | CL9583AP | 50ug |
Lympholyte®-poly Cell Separation Media (Isolation of PMNs from Human peripheral blood), sterile liquid | CL5071 | 250 mL |
Anti-Mouse PMN, Antiserum, (Polyclonal) (rabbit serum) | CLA31140 | 5mL |
Anti-Rat Myeloid Lineage, FITC (Clone OX82) (mouse IgG1) | CL081F | 100 ug |
Anti-Rat Macrophage, Adsorbed Antiserum, (Polyclonal) (rabbit serum) | CLAD51240 | 2mL |
Baby Rabbit Complement- Tissue Culture Grade, Frozen | CL3441-S50 | 50 ml |
Recombinant Human Gastrokine 3 | CLPRO2033-2 | 10ug |
Anti-Rat CD45RA/B FITC (Clone OX-33) (mouse IgG1) | CL033F | 100ug |
Mini Super PAP PEN (tip 1/10" diameter, 3 mL) | MU22 | EA |
Anti-Rat CD11b/c Purified (Clone OX-42) (mouse IgG2a) | CL042AP | 250ug |
Anti-Human Factor H (FITC) (Clone: OX-24) (mouse IgG1) | CL7672F | 100 ug |
Anti-Mouse I-Ad, Ascites (Clone 34-5-3S) (mouse IgG2a) | CL8713A | 0.5 mL |
Anti-Human Antithrombin III Affinity Purified (Polyclonal) (sheep IgG) | CL20015AP | 0.5mg |
Cedarlane/Anti-Rat CD45, 10 nm Gold Conjugate,OD3 (Clone OX-1) (mouse lgG1) | CL009G | 0.5 mL |
Cedarlane/Rat IL-2ELISA kit(precoated) | CL00113K-96 | 96T |