Avidity生物素蛋白连接酶-相关技术文章
时间：2024-07-11 阅读：89
Avidity生物素蛋白连接酶
Avidity LLC 成立于 1996 年，位于色彩缤纷的科罗拉多州。
生wu素化菌株；AviTag 载体；生wu素化重组蛋白我们AviTag™技术是利用大肠杆菌的生wu素连接酶(BIRA)，在一个du特的15个氨基酸肽标记上，对单一生wu素进行高度靶向的酶结合。AviTag™序列为GLNDIFEAKIEWHE。定向在链霉亲和素涂层表面，这创造了一个理想的表现分子结合相互作用。提供全套的蛋白、肽生wu素化的产品，其主要产品包括：生wu素蛋白连接酶（BirA）；生wu素化菌株；AviTag 载体；生wu素化重组蛋白。
以下是Avidity公司的部分热销产品
产品名称 | 货号 | 规格 |
BirA Biotin-Protein Ligase | BIRA500 | 40ug: 2 vials of 20ug each |
Bulk BirA Biotin-Protein Ligase | Bulk BIRA | 300ug: 10 vials of 30ug each |
Biotin Solution | BIO200 | 1.5 mL of 500uM d-biotin |
Biotin Solution | BIO500 | 10 mL of 5 mM d-biotin |
Positive Control Protein Substrate Kit | BIS300 | 1 Kit |
BIS-300 positive and negative control protein kit | BIS-300 | 1 kit |
Biotin AviTag™ Vectors (N-terminal) | pAN4 | 10 mg of plasmid DNA |
Chemically Competent E. coli B Strain AVB101 | CVB101 | 10 vials, 100 mL per vial |
Efficient Biotinylation E. coli K12 Strain AVB100 | AVB100 | 1 vial, containing 0.5 mL of glycerol stock |
Anti-C-terminus AviTag™-conjugated Agarose Resin | AbCA | 2.0 ml |
Efficient Biotinylation E. coli B Strain AVB101 | AVB101 | 1 vial, containing 0.5 mL of glycerol stock |
Electrocompetent E. coli B Strain AVB101 | EVB101 | 10 vials, 50 mL per vial |
Electrocompetent E. coli K12 Strain AVB100 | EVB100 | 10 vials, 50 mL per vial |
Positive Control Plasmid | BIP300 | 2ug |
Anti-C-terminus AviTag™ Antibody | AbC | 100ul, 1 mg/ml |
Biotinylated Gaussia princeps Luciferase | Gluc-10 | 10 ug |
Biotinylated Gaussia princeps Luciferase | Gluc-100 | 100 ug |
Streptavidin-conjugated Gaussia princeps Luciferase | SA-Gluc | 50 ug |
Native Coelenterazine Free Base | CTZ-1 | 1 mg |
Biotinylated Renilla reniformis Green Fluorescent Protein | rrGFP-100 | 100 ug |
Biotinylated Fluorescent Proteins(Twinkle-Avitag) | FP-495 | 100 ug |
Biotinylated Fluorescent Proteins(Holly-Avitag) | FP-515 | 100 ug |
Biotinylated Fluorescent Proteins(Dasher-Avitag ) | FP-525 | 100 ug |
Fluorescent Protein for detection of biotinylated molecules(SA-Twinkle) | SA-FP-495 | 100 ug |
Fluorescent Protein for detection of biotinylated molecules(SA-Holly) | SA-FP-515 | 100 ug |
Fluorescent Protein for detection of biotinylated molecules(SA-Cratchit) | SA-FP-545 | 100 ug |
Fluorescent Protein for detection of biotinylated molecules(SA-Yukon) | SA-FP-563 | 100 ug |
Fluorescent Protein for detection of biotinylated molecules(SA-Rudolph) | SA-FP-570 | 100 ug |
Biotin AviTag™ Vectors (N-terminal) | pAN4, 5, or 6 | 10ug of plasmid DNA |
Biotin AviTag™ Vectors (N-terminal) | pANs | one kit containing 10ug of each plasmid |
Biotin AviTag™ Vectors (C-terminal) | pAC4, 5, or 6 | 10ug of plasmid DNA |
Biotin AviTag™ Vectors (C-terminal) | pACs | one kit containing 10ug of each plasmid |
Forward-sequence primer for Biotin AviTag™ Vectors | FASP | 200 pmol |
Reverse-sequence primer for Biotin AviTag™ Vectors | RASP | 200 pmol |