人皮质酮CORT酶联免疫分析试剂盒实验原理：本试剂盒用于测定人血清，细胞上清及相关液体样本中皮质酮 (CORT)的含量。应用双抗体夹心法测定标本中人皮质酮 (CORT)水平。用纯化的人皮质酮 (CORT)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入皮质酮 (CORT)，再与HRP标记的皮质酮 (CORT)抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的人皮质酮 (CORT)呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中人皮质酮 (CORT)浓度。
Human Corticosterone (CORT) ELISA Kit
Principle of the assay
The kit assay Human CORT level in the sample，use Purified Human CORT to coat microtiter plate wells, make solid-phase antibody, then add CORT to wells, Combined antibody which With HRP labeled goat anti-Mouse become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of CORT in the samples is then determined by comparing the O.D. of the samples to the standard curve.
上海华壹生物科技有限公司
Shanghai HuaYi Bio-technology Co.,Ltd
