鱼雌激素ELISA试剂盒实验原理：本试剂盒用于测定鱼血清，血浆,组织及相关液体样本中激素（E）的含量。应用双抗体夹心法测定标本中鱼雌激素（E）水平。用纯化的鱼雌激素（E）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入雌激素（E），再与HRP标记的雌激素（E）抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的雌激素（E）呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中鱼雌激素（E）浓度。
Fish Estrogen(E)ELISA Kit
Purpose
This kit allows for the determination of E concentrations in Fish serum, blood plasma, tissue and other biological fluids.
Principle of the assay
The kit assay Fish E level in the sample，use Purified Fish E to coat microtiter plate wells, make solid-phase antibody, then add E to wells, Combined E antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of E in the samples is then determined by comparing the O.D. of the samples to the standard curve.
试剂盒供应商：上海华壹生物科技有限公司
