细胞凋亡检测试剂盒（AP ）
n 罗氏细胞检测系列产品自问世以来，历经时间的考验，以其的品质获得了广大业界用户的支持和认可，相关文献达数千篇。
细胞凋亡产品特性：
☆检测范围广泛：可实现对细胞培养液、组织切片、单个细胞及细胞群落的分析检测，满足日益增长的高通量检测需求。
☆检测方法灵活：针对光学或荧光显微镜、流式细胞仪、ELISA。
☆操作安全：无需接触任何放射性同位素，简便易用。
☆产品线丰富：针对不同的细胞凋亡通路，有多种产品可供挑选。
一、检测Caspase活性
1.Caspase-3活性
产品名及货号：Caspase 3 Activity Assay 12012952001
2.caspase对角蛋白18的裂解作用
产品名及货号：M30 CytoDEATH 12140349001
M30 CytoDEATH,Fluorescein 12156857001
3.Caspase对RARP的裂解作用
产品名及货号：Anti-Poly（ADP-Ribose）Polymerase（PARP） 11835238001
4.Caspase
产品名及货号：Homogeneous Caspase Assay,fluorimetric
二、检测DNA片断
①TUNEL检测法
产品名及货号：In Situ Cell Death Detection Kit,AP 11684809910
In Situ Cell Death Detection Kit,POD 11684817910
In Situ Cell Death Detection Kit,Fluorescein 11684795910
In Situ Cell Death Detection Kit,TMR red 12156792910
②Elisa检测
产品名及货号：Cell Death Detection Elisa 11774425001
③凝胶电泳检测
产品名及货号：Apoptotic DNA Ladder Kit 11835246001
三、检测膜变化
产品名及货号:Annexin-V-FLUOS 11828681001
Annexin-V-FLUOS Staining Kit 11988549001
Annexin-V-Alexa 568
Annexin-V-Biotin 11828690001
四、检测DNA合成
BrdU标记检测
产品名及货号：Celluar DNA Fragmentation ELISA 11585045001
ROCHE相关产品资料
In Situ Cell Death Detection Kit, AP
Kit for the detection and quantification of apoptotic cell death on a single-cell level by light microscopy in immunohistocytochemistry.
Catalog : 11684809910
Pack size:1 kit for up to 50 tests
Application
Qualitative detection of apoptosis at the single-cell level by light microscopy.
Benefits
- Sensitive: The maximum intensity of labeling （cell staining） of apoptotic cells is higher than the nick translation method
- Fast: The use of fluorescein-dUTP allows analysis of the samples directly after the TUNEL reaction, but before the addition of the secondary detection system
- Convenient: The direct labeling procedure using fluorescein-dUTP allows verification of the efficiency of the TUNEL reaction during the assay procedure
- Accurate: Identification of apoptosis at a molecular level （DNA-strand breaks） and identification of cells at the very early stages of apoptosis
- Flexible: No substrate included; provides the opportunity to select the staining procedure of choice
Product Description
Sample material: Cytospin and cell smear preparations, adherent cells grown on slides, and frozen and paraffin-embedded tissue sections.
Background Information
Widely used methods to determine apoptosis include the analysis of the genomic DNA by agarose-gel electrophoresis and DNA fragmentation assays based on 3H-thymidine and, alternatively, 5-Bromo-2'-deoxy-uridine. The methods involve the separation of fragmented, low molecular-weight DNA from unfragmented, high molecular-weight DNA in a given cell population. Thus, these methods do not provide information about the fate of an individual cell in a given cell population or, particularly, in tissue sections. Alternatively, individual apoptotic cells may be microscopically recognized because of the characteristic appearance of nuclear chromatin condensation and fragmentation, but this method is subjective and limited to a relatively narrow time window when the morphological changes are at a maximum.
The hallmark of apoptosis is DNA degradation, which in early stages is selective to the internucleosomal DNA linker regions. The DNA cleavage may yield double-stranded and single-stranded DNA breaks （nicks）. Both types of breaks can be detected by labeling the free 3'-OH termini with modified nucleotides （e.g., biotin-dUTP, DIG-dUTP, fluorescein-dUTP） in an enzymatic reaction. The enzyme terminal deoxynucleotidyl transferase （TdT） catalyzes the template-independent polymerization of deoxyribonucleotides to the 3'-end of single- and double-stranded DNA. This method has also been termed TUNEL （TdT-mediated dUTP-X nick end labeling）. Alternatively, free 3'-OH groups may be labeled using DNA polymerases by the template-dependent mechanism called nick translation. However, the TUNEL method is considered to be more sensitive and faster.
Contents
- Enzyme Solution （TdT）, 5 vials
- Label Solution （fluorescein-dUTP）, 5 vials
- Converter AP （anti-fluorescein antibody-AP）, ready-to-use
