小鼠黄体生成素（LH）ELISA试剂盒实验原理

小鼠黄体生成素(LH)ELISA试剂盒实验原理：用于测定小鼠血清，血浆及相关液体样本中黄体生成素(LH)含量。应用双抗体夹心法测定标本中小鼠黄体生成素(LH)水平。用纯化的小鼠黄体生成素(LH)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入黄体生成素(LH)，再与HRP 标记的黄体生成素(LH)抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物TMB 显色。TMB 在HRP 酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的黄体生成素(LH)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD 值），通过标准曲线计算样品中小鼠黄体生成素(LH)浓度。
Mouse luteinizing hormone(LH)ELISA Kit
Principle of the assay:
This kit allows for the determination of LH concentrations in Mouse serum, blood plasma,and other biological fluids.Assay Mouse LH level in the sample，use Purified Mouse LH antibody to coat microtiter plate wells, make solid-phase antibody, then add LH to wells, Combined LH which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of LH in the samples is then determined by comparing the O.D. of the samples to
the standard curve.
试剂盒供应商：上海华壹生物科技有限公司