Anti-PD-L1程序性死亡配体1抗体操作说明

Rabbit Anti-PD-L1 antibody 
 
Catalog Number : BYK-4941R        

Quantity size :   0.1ml  (1mg/ml   Buffer = 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol) 
 
Background: Programmed cell death 1 ligand 1(Programmed death ligand 1; PD-L1; PDCD1 ligand 1; B7 homolog 1; B7-H1; CD274) involved in the costimulatory signal, essential for T lymphocyte proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibit T-cell proliferation and cytokine production. 
 
Specificity :    

– Anti-PD-L1 is a rabbit Polyclonal antibody unconjugated                  

– specific for PD-L1 of Human, Mouse, Rat, Pig, Cow, Horse, Sheep

– use for western blotting,elisa,immunoprecipitation and immunohistochemistry

– Protein G affinity chromatography purification, purity :>95%

– Isotype:  IgG

– mol wt:  32kDa 
 
Application :                                    

– WB=1: 100-500  

– ELISA=1:500-1000  

– IHC-P=1:100-500  IHC-F=1:100-500  

– ICC=1:100-500  IF=1:100-500  

– Flow-Cyt=1:50-500

– Optimal working dilutions must be determined by the end user. 

 
Storage:  Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃ 
 
Important Note: This product as supplied is intended for research use only, not for use in human,therapeutic or diagnostic applications.