人尿微量白蛋白（mALB）ELISA试剂盒原理

人尿微量白蛋白(mALB）ELISA试剂盒
Human microalbunminuria(mALB)ELISA Kit
试剂盒原理：用于测定人尿液相关液体样本尿微量白蛋白(mALB）含量。本试剂盒应用双抗体夹心法测定标本中人尿微量蛋白(mALB)水平。用纯化的人尿微量蛋白(mALB)抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入尿微量蛋白
(mALB)，再与HRP 标记的mALB 抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物TMB 显色。TMB 在HRP 酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的尿微量蛋白(mALB)呈正相关。用酶标仪在450nm 波长下测定吸光度（OD 值），通过标准曲线计算样品中人尿微量蛋白(mALB)浓度。
Principle of the assay:
This kit allows for the determination of mALB concentrations in Human
Urine and other biological fluids.The kit assay Human mALB level in the sample，use Purified Human mALB antibody to coat microtiter plate wells, make solid-phase antibody, then add mALB to wells, Combined mALB antibody which With HRP labeled , become
antibody - antigen - enzyme-antibody complex, after washing Compley, Add
TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of mALB in the samples is then determined by comparing the O.D. of the samples to the standard curve.
试剂盒供应商：上海华壹生物科技有限公司