技术文章

罗氏试剂4709705001*

上海嵘崴达实业有限公司

2011/11/9 14:46:31

                                转染试剂4709705001*

德国罗氏试剂,部分常用现货,订货两到三周,每周三订单

www.runwelltac.com
http://www.biomart.cn/runwell/index.htm

转染试剂,货号:4709705001,品名:FuGENE HD Transfection Reagent,现货*,详情,上海嵘崴达,:

规格:1.0 ml up to 300 transfections

产品描述如下:

Composition: unique and proprietary blend of many components, in 80% ethanol.

存储: stable at +2°C to +8°C.

Stability: 2 years, starting from date of manufacture.

内容

Solution in 80% ethanol, filter-sterilized (0.1 µm filter), packaged in transparent glass vials.
 
质量

Lot-specific analysis:
Each lot of FuGENE®HD Transfection Reagent is carefully checked, using ISO DIN 9001-certified procedures, to assure product is performing according to specifications and is consistent from lot to lot.

Functional analysis:
Cos 7 cells are transfected in 96 well plates with a reporter gene vector DNA, using 0.3 µl FuGENE® HD and 0.1 µg DNA or 0.25 µl FuGENE® HD and 0.1 µg DNA. Reporter gene activity is monitored via a colorimetric test against a reference lot. Activity must be ≥ 75% compared to a reference lot in at least one of the tested amounts, listed above.

Cytotoxicity analysis:
Cell growth is assessed using the Cell Proliferation Reagent WST-1assay which is an indirect measure of cytotoxicity. The remainder of cells from functional analysis is tested for viability.

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