上海嵘崴达实业有限公司
2011/11/9 14:46:31转染试剂4709705001*
德国罗氏试剂,部分常用现货,订货两到三周,每周三订单
转染试剂,货号:4709705001,品名:FuGENE HD Transfection Reagent,现货*,详情,上海嵘崴达,:
规格:1.0 ml up to 300 transfections
产品描述如下:
Composition: unique and proprietary blend of many components, in 80% ethanol.
存储: stable at +2°C to +8°C.
Stability: 2 years, starting from date of manufacture.
Functional analysis:
Cos 7 cells are transfected in 96 well plates with a reporter gene vector DNA, using 0.3 µl FuGENE® HD and 0.1 µg DNA or 0.25 µl FuGENE® HD and 0.1 µg DNA. Reporter gene activity is monitored via a colorimetric test against a reference lot. Activity must be ≥ 75% compared to a reference lot in at least one of the tested amounts, listed above.
Cytotoxicity analysis:
Cell growth is assessed using the Cell Proliferation Reagent WST-1assay which is an indirect measure of cytotoxicity. The remainder of cells from functional analysis is tested for viability.