技术文章

Anti-CD25抗体说明书

上海研晶生化试剂有限公司

2012/2/7 9:13:29

Anti-CD25抗体说明书

 Quantity size:  0.2ml (dilute with pH 7.4    0.01 M PBS or diluent of antibody)
 
Background: CD25(IL2) receptor alpha (IL2RA) and beta (IL2RB) chains, together with the common gamma chain (IL2RG), constitute the high-affinity IL2 receptor. Homodimeric alpha chains(IL2RA) result in low-affinity receptor, while homodimeric beta (IL2RB) chains produce a medium-affinity receptor. Normally an integral-membrane protein, soluble IL2RA has been isolated and determined to result from extracellular proteolyisis. Alternay-spliced IL2RA mRNAs have been isolated, but the significance of each is presently unknown.
 Anti-CD25抗体说明书
Specificity:       ·anti- CD25 is a rabbit monoclonal antibody unconjugated
                ·specific for CD25 of rat  
                ·epitope mapping near the amino terminus of CD25 of  
                   rat origin
                · use for western blotting, elisa, immunoprecipitation    
                   and immunohistochemistry
· Protein A affinity chromatography purification, purity :>95%
· Isotype: IgG
· mol wt: 82kDa
 
Application:     · Western blotting: 1:100-500
· Immunohistochemistry: 1:100-500
· ELISA: 1:500-1000
· Optimal working dilutions must be determined by the end user.
 
Storage:   Store at –20 ºC for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20ºC. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 ºC.
 
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Anti-CD25抗体说明书

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