ESGRO和LIF在干细胞实验中的优点

LIF与ESGRO®用于干细胞培养的优势：

1.适合胚胎干细胞、神经干细胞、造血干细胞及间充质干细胞等多种干细胞培养

2.消除了饲养层细胞的干扰与污染

3.一致地抑制ES细胞的分化

4.减少了支原体污染的危险

5.方便使用的包装形式

Specific Activity:

ESGRO® is assessed both on ES {embryonic stem} cells （D3 & MBL-1） and on murine M1 myeloid leukemic cells（4）. A standard of 50 Units is defined as the concentration of ESGRO®, in 1.0mL of tissue culture medium, that induces the differentiation of 50% of M1 colonies（4）. 10E7 units is equivalent to approximay 100 μg of pure protein, and is sufficient to treat 10L of ES cell culture media.

Embryonic Stem Cell Assay: Differentiation inhibition at 1000 units/mL Murine myeloid leukemic, M1 Assay: Specific Activity >/= 108 units/mg

Promotional Text:

High Quality. Validated. Reliable.

EmbryoMax ESGRO® （LIF）

Description:

ESGRO® （LIF）

Trade Name:

Chemicon （Millipore）

Qty/Pk:107 units

Key Applications:

Stem Cell Culture

Application Notes:

Applications for ESGRO® include use as a reagent for the in vitro maintenance of the pluripotential phenotype of murine ES cells.

SUGGESTED PROTOCOLS:

ES Cells: In D3 and MBL-1 pluripotential ES cell cultures it is routinely found that 1000 U of ESGRO® per 1.0 mL of tissue culture media is required to maintain ES cells with a stem cell phenotype. Similar concentrations of mLIF have also been used for germline transmission of genetically altered ES cells（E3）.

At the recommended concentration 107 units of ESGRO® is sufficient for 10.0 L of tissue culture media and 106 units of ESGRO® is sufficient for 1.0 L of tissue culture media.

Visit www.esgro-lif.com for additional information