Rabbit Anti-11β-HSD2
FROM:ABCAM
Catalog Number : HZ982114
Quantity size : 0.2ml （dilute with pH 7.4 0.01 M PBS or antibody diluent ）
Background: Glucocorticoid hormone action in target tissues is modulated by 11β-hydroxysteroid dehydrogenase （11β-HSD）, which catalyzes the interconversion of hormonally active C11-hydroxylated corticosteroids （cortisol, corticosterone） and their inactive C11-keto metabolites （cortisone, 11-dehydrocorticosterone）. At least two isoforms of 11β-HSD exist a low-affinity NADP-dependent dehydrogenase/oxoreductase （11β-HSD2） and a high-affinity NAD-dependent dehydrogenase （11β-HSD2）. The glycosylated 11β-HSD2 protein activates cortisol from cortisone, which is widely expressed in mammals, and is most highly expressed in the liver . 11β-HSD2 inactivates cortisol to cortisone and is expressed in placenta, aldosterone target tissues （kidney, parotid, colon and skin） and pancreas. 11β-HSD2 may play a role in glucose homeostasis and pathogenesis of a number of disorders including insulin resistance and obesity. 11β-HSD2 associates with differentiation or maturation in human colonic epithelia and may serve as a marker in development and disease. In addition, 11β-HSD2 plays a crucial role in modulating mineralcorticoid and glucocorticoid receptor occupancy by glucocorticoids.
Rabbit Anti-11β-HSD2 pecificity :
– Anti- 11β-HSD2 is a rabbit monoclonal antibody unconjugated
– specific for 11β-HSD2 of human, mouse, rat
– use for western blotting,elisa,immunoprecipitation and immunohistochemistry
– Protein G affinity chromatography purification, purity :>95%
– Isotype: IgG
– mol wt: 34kDa
Application :
– Western blotting 1:100-500
– Immunohistochemistry 1:100-500
– ELISA 1:500-1000
– Optimal working dilutions must be determined by the end user.
Storage: Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Rabbit Anti-11β-HSD2
