大鼠8异前列腺素（8-iso-PG）ELISA试剂盒实验原理

大鼠8异前列腺素（8-iso-PG）酶联免疫分析试剂盒仅供研究使用，用于测定大鼠血清，血浆及相关液体样本中8-异前列腺素（8-iso-PG）的含量，实验原理：应用双抗体夹心法测定标本中大鼠8-异前列腺素（8-iso-PG）水平。用纯化的8-异前列腺素（8-iso-PG）抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入大鼠8-异前列腺素（8-iso-PG），再与HRP标记的8-异前列腺素 （8-iso-PG）抗体结合，形成抗体-抗原-酶标抗体复合物，经过*洗涤后加底物TMB显色。TMB在HRP酶的催化下转化成蓝色，并在酸的作用下转化成zui终的黄色。颜色的深浅和样品中的8-异前列腺素（8-iso-PG）呈正相关。用酶标仪在450nm波长下测定吸光度（OD值），通过标准曲线计算样品中大鼠8-异前列腺素（8-iso-PG）浓度。
Drug Names
Generic Name：Rat 8-iso prostaglandin（8-iso-PG） ELISA Kit.
Purpose
This kit allows for the determination of 8-iso-PG concentrations in Rat serum, blood plasma, and other biological fluids.
Principle of the assay
The kit assay Rat 8-iso-PG level in the sample，use Purified Rat 8-iso-PG to coat microtiter plate wells, make solid-phase antibody, then add 8-iso-PG to wells, Combined 8-iso-PG antibody which With HRP labeled , become antibody - antigen - enzyme-antibody complex, after washing Compley, Add TMB substrate solution,TMB substrate becomes blue color At HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of 8-iso-PG in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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