技术文章

Recombinant Human Basic Fibroblast Growth Factor

Catalog Number: Ts-C204-02
Source: Escherichia coli.
Molecular Weight: Approximay 16.5 kDa, a single non-glycosylated polypeptide chain containing 147 amino acids.
Quantity: 10µg/50µg/1000µg
AA Sequence: MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP
HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE
SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS
Purity: > 96 % by SDS-PAGE and HPLC analyses.
Biological Activity: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.05 ng/ml, corresponding to a specific activity of >2.0 × 107 IU/mg.
Physical Appearance: Sterile Filtered White lyophilized （freeze-dried） powder.
Formulation: Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
Endotoxin: Less than 1 EU/µg of rHubFGF as determined by LAL method.
Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
Storage: This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage,preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles.
Usage: This material is offered by Shanghai PrimeGene Bio-Tech for research, laboratory or further evaluation purposes. NOT FOR HUMAN USE.
Human Basic Fibroblast Growth Factor
Human bFGF, encoded by the FGF2 gene, is a member of the fibroblast growth factor （FGF） family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further
fractionating the extract using acidic and basic pH, two different forms have isolated that named "acidic fibroblast growth factor"（FGF-1） and "basic fibroblast growth factor" （FGF-2）. Human bFGF shares 54 % amino acid sequence identity with aFGF.Affinity between bFGF and its receptors can be increased by heparin or heparan sulfate proteoglycan. bFGF plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. bFGF are also involved in a variety of biological processes, including embryonic development , morphogenesis, tissue repair, tumor growth and invasion.
Additionally, bFGF is frequently used for a critical component of cell culture medium, e.g., human embryonic stem cell culture medium, serum-free culture systems.