ORAI1/FITC标记抗体说明书

Anti-ORAI1/FITC标记抗体说明书

From:ABCAM

Catalog Number :RS-9571R-FITC

Quantity size :   0.1ml  （dilute with pH 7.4    0.01 M PBS or antibody diluent ） Background: Annexin V （ANX5） is a member of a family of calcium-dependent phospholipid binding proteins. This family of annexins also includes giardin that has been shown to function as an annexin; pfam00191
 

Orai1 is a 254 amino acid multi-pass membrane protein that belongs to the orai family of proteins. Localizing to the plasma membrane, Orai1 plays an important role in store-operated calcium （SOC） entry, a process involving Ca2+ influx and replenishment of Ca2+ stores formerly emptied through the action of inositol 1,4,5-trisphosphate production and other Ca2+ mobilizing agents. CRAC channels are responsible for medi-ating calcium influx in T-cells and play an important role in the immune response. Orai1 specifically increases the Ca2+-selective current through coaction with the Ca2+ sensor Stim1.

Specificity : Anti- ORAI1/FITC a rabbit polyclonal antibody             

specific for ORAI1 /FITC of  Human,Mouse,Rat,sheep,

• use for western blotting,elisa,immunoprecipitation and immunohistochemistry
• Protein G affinity chromatography purification, purity :>95%
• Isotype:  IgG
• mol wt:  29kDa

Application :  

• Flow-Cyt=1:50-200  IF=1:50-200
• Excitation spectrum:490-495nm
• Emission spectrum:520-530nm
• not yet tested in other applications.
  optimal dilutions/concentrations should be determined by the end user.

Storage:  Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The  lyophilized antibody is stable at room temperature for at least one month and for greater  than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent  of antibody, the antibody is stable for at least six weeks at 2-4 ℃

Important Note: This product as supplied is intended for research use only, not for use in  human, therapeutic or diagnostic applications.