上海圻明生物科技有限公司
2013/10/9 8:46:12Rabbit Anti-NSE/ENO2/FITC
Quantity size: 100ug （2mg/1ml, Lyophilized or Liquid. Buffer = 0.01M PBS, pH 7.4 with 10 mg/ml BSA and 0.1% Sodium azide. Reconstitute with 50ul sterile distilled water.）
Background: This gene encodes one of the three enolase isoenzymes found in mammals. This isoenzyme, a homodimer, is found in mature neurons and cells of neuronal origin. A switch from alpha enolase to gamma enolase occurs in neural tissue during development in rats and primates. SUBUNIT: Mammalian enolase is composed of 3 isozyme subunits, alpha, beta and gamma, which can form homodimers or heterodimers which are cell-type and development-specific. Subcellular location: Cytoplasm （By similarity）. Cell membrane （By similarity）. Note=Can translocate to the plasma membrane in either the homodimeric （alpha/alpha） or heterodimeric （alpha/gamma） form （By similarity）. TISSUE SPECIFICITY: The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.developmental stage: During ontogenesis, there is a transition from the alpha/alpha homodimer to the alpha/beta heterodimer in striated muscle cells, and to the alpha/gamma heterodimer in nerve cells. Belongs to the enolase family.
Also known as: neuron specific enolase; 2 phospho D glycerate hydrolyase; Eno 2; Eno2; ENOG; Enolase 2; Enolase 2 gamma neuronal; Enolase2; Gamma enolase; Neural enolase; Neuron specific enolase; Neuron specific gamma enolase; Neurone specific enolase; NSE.
Specificity:
l Rabbit Polyclonal IgG, affinity purified by Protein A.
l FITC conjugated
l Reacts with: hu, mo, rat
l Protein G affinity chromatography purification, purity:>95%
Application: FCM:1:20-100
Immunohistochemistry:1:20-200
Immunocytochemistry:1:20-200
Excitation spectrum: 490-495nm
Emission spectrum: 520-530nm
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.
Storage: Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The Lyophilized or Liquid antibody is stable at room temperature for at least one month and for greater than a year when kept at -20 °C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
