技术文章

上海圻明生物科技有限公司
SHANGHAI QiMing BIOTECHNOLOGY CO.，LTD.
Rabbit Anti-ATG3
Cat. Number: qm-01821
Quantity size: 0.1ml （1mg/1ml, Lyophilized or Liquid. Buffer = 0.01M PBS, pH 7.4 with 10
mg/ml BSA and 0.1% Sodium azide. Reconstitute with 0.1ml sterile distilled water.）
Background: E2-like enzyme involved in autophagy and mitochondrial homeostasis. Catalyzes
the conjugation of ATG8-like proteins （GABARAP, GABARAPL1, GABARAPL2 or
MAP1LC3A） to phosphatidylethanolamine （PE）. PE-conjugation to ATG8-like proteins is
essential for autophagy. Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like
enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role
in mitochondrial homeostasis but not in autophagy. ATG7 （E1-like enzyme） facilitates this
reaction by forming an E1-E2 complex with ATG3.
Also known as: 2610016C12Rik; Apg 3; APG3; APG3 autophagy 3 like; APG3 like; APG3-like;
APG3L; Apg3p; ATG 3; ATG3; ATG3 autophagy related 3 homolog; ATG3 autophagy related 3
homolog （S. cerevisiae）; ATG3_HUMAN; Autophagy Apg3p/Aut1p like; Autophagy related
protein 3; Autophagy-related protein 3; DKFZp564M1178; FLJ22125; hApg3; MGC15201;
OTTHUMP00000214547; OTTHUMP00000214548; PC3 96; Protein PC3-96;
Ubiquitin-like-conjugating enzyme ATG3.
Specificity:
 Rabbit Polyclonal IgG, affinity purified by Protein A.
 Reacts with: Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit,
 Immunogen: KLH conjugated synthetic peptide derived from human ATG3.
 Predicted molecular weight: 36kDa
Application: WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500
IF=1:100-500
Not yet tested in other applications.
Optimal working dilutions must be determined by the end user.
Storage: Store at -20 ° C for one year. Avoid repeated freeze/thaw cycles. The Lyophilized or
Liquid antibody is stable at room temperature for at least one month and for greater than a year
when kept at -20 °C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the
antibody is stable for at least two weeks at 2-4 °C.
Important Note: This product as supplied is intended for research use only, not for use in human,
therapeutic or diagnostic applications.