英文名称:Xanthine Oxidase from bovine milk;XOD
CAS号:9002-17-9
分子量:181000
类型:Grade I
活力:0.4-1.0 units/mg protein
活力定义:One unit will convert 1.0 μmole of xanthine to uric acid per min at pH 7.5 at 25 °C. Approx. 50% of the activity is obtained with hypoxanthine as substrate
uricase :≤0.5%
性状:液体。能溶于水。广泛存在于牛奶、肝脏、牛小肠和细菌等生物资源中,主要从牛奶中提取而得。zui适pH值为8.3,pI5.3~5.4,主要抑制剂有Hg2+,Ag+,Cd2+及尿素、氰化物、磷酸根和氯离子,主要稳定剂有水杨酸盐、半胱氨酸、组胺和维尔烯酸盐。
用途:生化研究。它是一种催化黄嘌呤、次黄嘌呤生成尿酸的酶,它常作工具酶。用于黄嘌呤、次黄嘌呤、肌苷、鸟嘌呤、鸟苷等的测定
保存:2~8℃
英文名称:Xanthine Oxidase from bovine milk;XOD
CAS号:9002-17-9
分子量:160000(gel)
活力:≥90units/mg protein
活力定义:One unit will cause the formation of one micromole of uric acid per min at pH73.5 at 37℃
Isoelectic point:4.0
PH stability:6.0~9.5
Optimum pH:7.0~7.5
Optimum temperature:55℃
Thermal stability:≤55℃(PH7.5, 20 min)
Preparation Instructions:the enzyme is reconstituted in 50mM potassium phosphate buffer,pH7.5 for activity assay
性状:能溶于水。广泛存在于牛奶、肝脏、牛小肠和细菌等生物资源中,主要从牛奶中提取而得。主要抑制剂有Hg2+,Ag+,Cd2+及尿素、氰化物、磷酸根和氯离子,主要稳定剂有水杨酸盐、半胱氨酸、组胺和维尔烯酸盐。
用途:生化研究。它是一种催化黄嘌呤、次黄嘌呤生成尿酸的酶,它常作工具酶。用于黄嘌呤、次黄嘌呤、肌苷、鸟嘌呤、鸟苷等的测定
保存:2~8℃