Designations:

143B

Depositors:

 Wistar Institute

Biosafety Level:

1

Shipped:

frozen

Medium & Serum:

See Propagation

Growth Properties:

adherent

Organism:

Homo sapiens

Morphology:

mixed

 

Source:

Organ: bone 
Disease: osteosarcoma

Permits/Forms:

In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications:

transfection host

DNA Profile (STR):

Amelogenin: X 
CSF1PO: 12 
D13S317: 12 
D16S539: 10,13 
D5S818: 13 
D7S820: 11,12 
THO1: 6 
TPOX: 11 
vWA: 18

Age:

13 year old

Gender:

female

Ethnicity:

Caucasian

Comments:

Thymidine kinase negative (TK-). 
This is a human osteosarcoma cell line.

Propagation:

ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with 0.015 mg/ml 5-bromo-2'-deoxyuridine, 90%; fetal bovine serum, 10%
Temperature: 37.0°C

Subculturing:

Medium Renewal: 2 to 3 times per week 
Remove medium, rinse with fresh 0.25% trypsin, 0.02% EDTA solution and allow the cells to sit at room temperature (or at 37C) until they detach (about 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Preservation:

90% FBS; 10% DMSO

Related Products:

derivative:ATCC CRL-8304

References:

32372: Berson JF, et al. A seven-transmembrane domain receptor involved in fusion and entry of T-cell-tropic human immunodeficiency virus tyep 1 strains. J. Virol. 70: 6288-6295, 1996. PubMed: 870925632519: Roller RJ, et al. Structure and function in the herpes simplex virus 1 RNA-binding protein US11: mapping of the domain required for ribosomal and nucleolar association and RNA binding in vitro. J. Virol. 70: 2842-2851, 1996. PubMed: 862775833047: Hofhaus G, et al. Respiration and growth defects in transmitochondrial cell lines carrying the 11778 mutation associated with Leber's hereditary optic neuropathy. J. Biol. Chem. 271: 13155-13161, 1996. PubMed: 866275733152: Hocking AM, et al. Eukaryotic expression of recombinant biglycan. J. Biol. Chem. 271: 19571-19577, 1996.