起订量:
ICP0603 *基赖氨酸抗体FITC标记Trimethyl Lysine Antibody ,FITC
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代理商南宁市蓝光生物技术有限公司成立于2008年,坐落在广西南宁市*开发区。是一家由活跃在美国、加拿大生物科技前沿的华人科学家和留学生创建的。其核心技术及研发平台由加拿大ICP免疫公司和美国、加拿大数家拥有*科学家和设备的实验室提供。
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Product Description | This rabbit polyclonal antibody is raised with a chemically methylated protein antigen. The specific anti-tri-methyl-lysine antibody was affinity purified with trimethylated lysine antibodies and affinity adsorbed with N-(epsilon) mono- and di-methylated lysine. The purified anti-trimethyl-lysine antibody was labeled with FITC. The antibody could be utilized for detection and localization of proteins with N-trimethylated lysine residues. |
| Immunofluorescent staining of paraformaldehyde fixed human melanoma cells (MMRU) with ICP0603 (5 µg/mL in PBSt, 3% BSA, r.t, 30 min) |
Formulation | 250 µg/mL in PBS with 50% glycerol | ||
Immunogen | Methylated KLH conjugates | ||
Purification | The antibody was purified on N (epsilon) tri-methyl lysine agarose and absorbed by mono- and di-methyl lysine. | ||
Conjugation | Flourescein 5(6) isothionate; FITC/Ab molar ratio - >20:1 | ||
Specificity | The antibody recognizes proteins with trimethylation on their lysine residues (N-epsilon). There are no cross reactions with acetylated proteins or mono- and di-methylated proteins. | ||
Species Tested | Human and mouse | ||
Applications | IF | ||
Scientific Description | Trimethylation of the epsilon amino group of lysine residues in proteins is one of the most important events of post-translational modification. | ||
Storage & Stability | Product is stable for several weeks at 4°C. For extended storage, store product at -20°C. Expiration date is one year from date of shipping if stored properly. |