- 面议
起订量：
IF9204 MOU-IP Anti-mouse IgG (HRP) for IP/CoIP
- 型号
- IF9204
上海维景生物科技有限公司是一家专注生命科学领域的企业，拥有强大的资金背景支持和专业的团队等资源。公司面向全国市场，迅速发展，广纳英才，现已建成专业生命科学产品销售团队，其中大部份是在生命科学市场上工作多年，有丰富的销售经验，也有良好的客户资源，销售渠道全面覆盖全国范围大部分工业以及科研客户，团队中免疫学及相关专业硕士以上学历人士达80%，有较强的技术支持能力，在针对专业技术服务中能够提供完善周到的专业化服务，对客户在使用产品过程中提出的问题能满意答复，并可及时传递较早的产品技术资料信息。
上海维景生物是一家面向全国以专业从事免疫学实验试剂和抗体试剂代理销售为主的公司，服务对象主要为大学研究所科研人员、大型制药公司等。
同时公司还是美国Engibody品牌的全国总代理，Engibody公司产品专注于标签抗体、内参抗体及标签抗体偶联beads这一领域。其开发的标签抗体偶联agarose beads（affinity gel）在科研以及临床研究中享有很高的声誉，更借助Engibody在标签抗体、内参抗体领域的优势，成为WB,IP,Co-IP全套试剂及试剂盒的专业供应商。
维景生物是一家专注标签抗体偶联beads（affinity gel），标签抗体，HRP直标标签抗体，内参抗体等抗体销售,及WB,IP,Co-IP全套试剂及试剂盒的高科技生物公司。
详细信息
产品名称：MOU-IP Anti-mouse IgG (HRP) for IP/CoIP
货号： IF9204
品牌：Engibody
简介：IP专用抗小鼠二抗， CoIP专用抗小鼠二抗 ，用于下游WB实验中，消除免疫沉淀抗体的轻重链干扰
MOU-IP™ Anti-mouse IgG for IP (HRP Conjugated) secondary antibody is western blotting reagents that enable the trouble-free detection of western blotted target protein bands from upstream IP/CoIP, without interference from denatured IgG (IP primary antibody). This allows to detect the (co-)immunoprecipitated protein without interfering by the IgG heavy (50 kDa) and light chains (25 kDa). In general, this interference tends to originate from traditional secondary antibodies which recognize primary antibodies released with the antigen during the immunoprecipitation procedure or endogenous IgGs from the lysate itself.
MOU-IP™ Anti-mouse IgG for IP (HRP Conjugated) only recognize native (non-reduced) primary antibodies (from mouse) and therefore the bands of heavy and light chains is highly minimized, if the immunoprecipitated antigen-antibody complex is fully reduced and denatured before downstream WB.
Description
MOU-IP™ Anti-mouse IgG (native conformation antibody specific) for IP (HRP Conjugated)
Reactivity
Mouse
Tested applications
WB : 1/1000-1/2000. Optimal dilutions should be determined by the end user.
Specificity
This secondary antibody is specific to native mouse polyclonal/monoclonal antibody
Immunogen
The antibody was developed in goat using the nature mouse IgG as the immunogen and then develop monoclonal antibody.
Clonality
Monoclonal, clone number: 7G9
Isotype
Goat IgG
Form
The antibody in 10 mM PBS, pH 7.4, 50% glycerol with 10 mg/mL (1% w/v) BSA as a stabilizer and 0.01% (w/v) thimerosal as preservative.
Storage instruction
Store at -20°C, Avoid freeze / thaw cycle. Do not aliquot the antibody.
Conjugation
Horseradish Peroxidase (HRP)
Host
Goat
MOU-IP™ IP专用的抗小鼠IgG二抗（天然构象完整分子抗体特异性）（HRP偶联）是一种蛋白IP-WB试剂，其能够无障碍地检测来自上游IP/CoIP的蛋白质印迹目标蛋白带，而不受变性IgG的干扰（IP一抗）。这允许在不受IgG重链（50kDa）和轻链（25kDa）干扰的情况下检测（共）免疫沉淀蛋白。通常，这种干扰倾向于源于传统的二抗，其识别在免疫沉淀过程中与抗原一起释放的一抗或来自裂解物本身的内源性IgG的轻链和重链。
MOU-IP™ 如果免疫沉淀的抗原-抗体复合物在下游WB之前被还原和变性，则用于IP的抗小鼠IgG（HRP标记）仅识别天然（非还原）一抗（来自小鼠），因此重链和轻链的条带不会显示。
描述
MOU-IP™ IP专用的抗小鼠IgG二抗（天然构象完整分子抗体特异性）（HRP偶联）
反应性
识别小鼠完整抗体分子
经过测试的应用实验
WB:1/1000-1/2000。最佳稀释度应由最终用户确定。
特异性
该二抗对天然小鼠多克隆/单克隆抗体完整分子具有特异性识别，同时不识别小鼠抗体单独轻重链
免疫原
以天然小鼠IgG为免疫原，在山羊身上制备抗体，然后制备只识别小鼠抗体完整分子同时不识别小鼠抗体单独轻重链的单克隆抗体。
克隆性
单克隆，克隆号：7G9
同型对照
山羊IgG
类型
抗体在10 mM PBS中，pH 7.4，50%甘油，10 mg/mL（1%w/v）BSA作为稳定剂，0.01%（w/v）硫柳汞作为防腐剂。
存储说明
储存于-20°C，避免冷冻/解冻循环。不要分装抗体。
偶联标记
辣根过氧化物酶（HRP）
抗体来源宿主
山羊
Figure 1. MOU-IP™ Anti-mouse IgG for IP (HRP) secondary antibody images: Western blotting.
IP sample preparation:
target protein (PCNA) was immunoprecipitated from 0.5 mL cell lysate of 1x107 Jurkat cells with 5 µg anti-human PCNA mouse monoclonal antibody and protein A/G agarose beads (Engibody, IF0001).
WB conditions:
the immunoprecipitated antigen-antibody complex should be boiled for 5-10 minutes in SDS sample buffer with a increase in SDS amount if required, make sure that the complex is fully reduced and denatured before downstream WB.
then electrophoresis, transferred to a PVDF membrane, and incubate with an anti- PCNA mouse monoclonal antibody.
Secondary Antibody Detection:
Lane 1: Detection with MOU-IP™ Anti-mouse IgG for IP (HRP) (CAT: IF9204)
The heavy and light chains can not be seen, confirming that although the immunoprecipitating heavy and light-chains are present, detects only native antibody and not denatured heavy and light-chains.
Lane 2: Detection with a traditional goat anti-mouse IgG (H&L) (HRP) secondary antibody (CAT: AT0098)
Figure 2. A schematic representation of IP-WB workflow by using MOU-IP™ secondary antibody.
Using primary antibody coupled beads
MOU-IP™ Anti-mouse IgG for IP (HRP Conjugated) only recognize native (non-reduced) primary antibodies (from mouse) and therefore the bands of heavy chain and light chain are highly minimized, if the immunoprecipitated antigen-antibody complex is fully reduced and denatured before downstream WB.
Figure 3. A schematic representation of IP-WB workflow by using MOU-IP™ secondary antibody.
Using protein A/G Beads + primary antibody
MOU-IP™ Anti-mouse IgG for IP (HRP Conjugated) only recognize native (non-reduced) primary antibodies (from mouse) and therefore the bands of heavy chain and light chain are highly minimized, if the immunoprecipitated antigen-antibody complex is fully reduced and denatured before downstream WB.
