起订量:
Mito-RealThiol (MitoRT)线粒体 (GSH) 探针
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代理商ClodronateLiposomes.com.cn是靶点科技(北京)有限公司(Target Technology (Beijing) Co.,Ltd.)旗下专注于单核巨噬细胞系统的专业性门户网站。
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平台在强化“Bench-to-Bedside”基础产品开发的同时,为了更好的给目标客户赋能,提供常用大小鼠模拟的人类疾病模型的构建服务,同时,不定期举行实验性的主题培训班,让客户能获得自己独立动手的实验能力。此外,平台出版免费电子期刊,以促进实验和文献的交流共享。
平台团队对单核巨噬细胞系统有着深刻的认识和理解,从分离,富集,鉴定,示踪,培养和细胞清除提供一整套综合解决方案。专业的技术支持团队,确保能够为客户给予专业的售前咨询和售后服务。
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Mito RealThiol (MitoRT) provides ratiometric fluorescent readout for quantification of mitochondrial GSH in live mammalian cells.
Highlights:
- MitoRT is cell permeable and preferentially accumulated in mitochondria
- Conveniently compare mitochondrial GSH levels in live cells using confocal microscopy, and FACS
- Signals from blue and green channels allow for deduction of mitochondrial GSH concentration, given an appropriate calibration curve
Subcellular detection and localization of GSH is important in understanding the modulation of redox status, the effect of drugs, and the mechanisms of detoxification. Furthermore, differences in GSH levels in response to oxidative stress in subpopulations of cells have been reported, underscoring the importance of detection methods amenable to flow cytometry and automated fluorescence detection.
Product Type: | Small Molecule |
Name: | Mito-RealThiol (MitoRT) |
Chemical Formula: | C38H35BrN3O3P |
Molecular Weight: | 692.59 |
Format: | Red to orange solid (Lyophilized powder in tubes) |
Purity: | >97%, HPLC |
Tested Applications: | 1 µM for cell staining |
Solubility: | DI Water (DO NOT dissolve in DMSO) |
Spectral Information: | Ex/Em: MitoRT: 488/565; MitoRT-GSH: 405/488 |
Platform: | Confocal microscopy/FACS |
Compatible Cells: | Mammalian cells e.g. HeLa, HT1080 |
Detection Method: | Fluorescence |
Comments: | - Dilute stock with imaging buffer. - A laser-based light source is preferred for best results. |
Storage: | -80C |
Shipped: | Ambient Temperature |