- 面议
起订量：
11277081001-- Hexanucleotide Mix
- 型号
- 11277081001--
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详细信息
Application
Use the Hexanucleotide Mix to prime cDNA synthesis, e.g. for:
- RT-PCR
- Generation of cDNA libraries
- Synthesis of first-strand cDNA
Product Description
Composition: The product is a 10x concentrated mixture of random hexanucleotides. Statistically, the mix may contain up to 4096 different hexanucleotides, but these are probably present in differing amounts.
Synthesis: All 4 bases are used to synthesize this random hexanucleotide mix. In the initial reaction, starter nucleotides are linked to a solid phase support. In subsequent coupling reactions, equimolar amounts of the 4 dNTPs are linked to the starter nucleotides until hexamers are generated. The hexamers are then released from the solid phase support.
Post-synthesis: The oligonucleotides are HPLC purified, desalted, and 5’-phosphorylated.
Contents
10x working solution of p（dN6） （62.5 A260/ml） in reaction buffer.
Additional Information
Special Interest Sites
- Innovative Tools for Amplification
Product Literature
- PCR Manual
Instructions for Use and Material Safety Data Sheets
- Catalog Number:11277081001
Tools
- LABfaqs "Find a Quick Solution", 3rd edition
Associated Products
- mRNA Capture Kit;
- Random Primed DNA Labeling Kit;
For labeling DNA using random oligonucelotides as primers - High Pure RNA Isolation Kit;
Low to medium throuhgput, mini scale RNA isolation. - mRNA Isolation Kit;
- Quick Spin Columns for radiolabeled DNA purification Sephadex G-50, fine;
- TriPure Isolation Reagent;
- Protector RNase Inhibitor;
Special Quality for Molecular Biology. - FastStart High Fidelity PCR System, dNTPack;
FastStart High Fidelity Enzyme Blend with ready-to-use PCR Nucleotide Mix for hot start PCR and amplification of genomic and cDNA targets up to 5 kb. - FastStart High Fidelity PCR System;
Blend of FastStart Taq DNA Polymerase and a thermostable proofreading protein for high fidelity hot start PCR of DNA fragments up to 5 kb. - PCR Nucleotide Mix;
Highest purity nucleotides for ambitious applications like PCR. - DIG DNA Labeling and Detection Kit;
For random-primed DNA labeling with digoxigenin-dUTP, alkali-labile and detection of hybrids by enzyme immunoassay. - Transcriptor Reverse Transcriptase;
A recombinant reverse transcriptase for the robust transcription of RNA fragments up to 14 kb used in two-step RT-PCR on thermal cyclers and real-time PCR instruments. - DIG DNA Labeling Kit;
- Primer for cDNA Synthesis;
- High Pure RNA Tissue Kit;
Low to medium throughput RNA isolation.
