产地：Roche
货号：11168860001
Quality
Absence of nonspecific endonuclease activities
1 μg λDNA is incubated for 16 h in 50 μl SuRE/Cut buffer B with an excess of Bbr PI. The number of enzyme units that may be included in the incubation mixture without changing the enzyme-specific digestion pattern is stated under "Endo" （on the product label）.
Absence of exonuclease activity
Approx. 5 μg [3H] labeled calf thymus DNA are incubated with 3 μl Bbr PIfor 4 h at 37°C in a total volume of 100 μl 50 mM Tris-HCl, 10 mM MgCl2, 1 mM dithioerythritol, pH approx. 7.5. The amount of radioactivity released is calculated as a percentage of input radioactivity, then divided by the number of units of enzyme. That calculated value is stated under "Exo" （on the product label）.
Contents
Bbr PI
SuRE/Cut buffer B （10x）
Product Description
Sequence specificity
Bbr Pl recognizes the sequence CAC↓GTG and generates fragments with blunt ends.
Source
From Bacillus brevis
Compatible ends
Bbr Pl ends are compatible with any blunt end.
Isoschizomers
BbrP I is an isoschizomer of Eco 72I, Pma C I and Pml l.
Methylation sensitivity
No information available.