sciencell  内皮细胞培养基（ECM）

Endothelial Cell Medium(ECM)

Catalog Number: 1001

Product Description

Endothelial cell medium (ECM) is a complete medium designed for optimal growth of normal human  microvascular endothelial  cells  in vitro.  It is a sterile, liquid medium  which  contains essential and non-essential amino acids, vitamins, organic and inorganic compounds, hormones, growth factors, trace minerals and a low concentration of fetal bovine serum (5%). The medium is bicarbonate buffered and has a pH of 7.4 when equilibrated in  an incubator with an atmosphere of 5% CO2/95% air. The medium is formulated (quantitatively and qualitatively) to provide a defined and optimally balanced nutritional environment that selectively promotes proliferation and growth of normal human microvascular endothelial cells in vitro.

Components

ECM consists of 500 ml of basal medium, 25 ml of fetal bovine serum (FBS, Cat. No. 0025), 5 ml of  endothelial cell growth supplement (ECGS, Cat. No. 1052) and 5 ml of  penicillin/streptomycin solution (P/S, Cat. No. 0503). 

Product Use

ECM is for research use only.  It is not approved for human or animal use, or for application in in vitro diagnostic procedures.

Storage

Store the basal medium at 4℃, the ECGS, the FBS and the P/S solution at -20℃. Protect from light. 

Shipping

Gel ice.

Prepare for use

Thaw  ECGS, FBS and P/S solution at 37℃.  Gently tilt the  ECGS tube several times during thawing  to help the contents dissolve.  Make sure the contents of the supplement are compley dissolved into solution before adding to the medium. Rinse the bottle and tubes with 70% ethanol, and then wipe to remove excess. Remove the cap, being careful not to touch the interior threads with fingers. Add ECGS, FBS and P/S solution into basal medium in a sterile field, mix well and then the reconstituted medium is ready for use. Since several components of this medium are light-labile, it is recommended that the medium not be exposed to light for lengthy periods of time. If the medium is warmed prior to use, do not exceed 37℃. When stored in the dark at 4℃, the reconstituted medium is stable for one month.

Caution:

If handled improperly, some components of the medium may present a health hazard. Take appropriate precautions when handling it, including the wearing of protective clothing and eyewear. Dispose of properly.

美国ScienCell研究实验室成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力于实验室科研用原代细胞、原代细胞培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发，在拥有众多客户。在国内销售8年来，很多老师应用其产品发表了高质量的SCI文章，且有着的文献引用率。凭借着严格的质控和的产品品质，深受广大科研工作者的信赖。
       ScienCell研究实验室生产的原代细胞、原代细胞培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正常细胞系统，90多种不同细胞类型。大多数细胞在唯有ScienCell实验室能够成功分离，产品质量过硬。确保了实验结果的重复性和连贯性。

内皮细胞培养基（ECM）是专门为正常人类微血管内皮细胞体外培养设计的*适于其生长的*培养基。内皮细胞培养基（ECM）是经灭菌的液体培养基，包含必需和非必需氨基酸、维生素、有机和无机化合物、***、生长因子、微量矿物质和低浓度胎牛血清(5%)。内皮细胞培养基（ECM）缓冲体系为重碳酸盐，在含5%CO2的细胞培养箱中平衡后pH值为7.4。内皮细胞培养基（ECM）的配方能够选择性的促进正常人类微血管内皮细胞体外培养中的增殖和生长，并为其达到*理想营养平衡状态提供数量上和质量上的保证。
      内皮细胞培养基（ECM）包含500ml基础培养基，25ml胎牛血清(FBS,目录编号0025)，5ml内皮细胞生长添加物(ECGS,目录编号1052)，和5 ml青霉素/链霉素溶液(P/S,目录编号0503)

sciencell  内皮细胞培养基（ECM）订购说明：

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sciencell  内皮细胞培养基（ECM）运输说明：

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