如果采用前述前处理方法对生物样品进行处理后,依然发现目标组分受干扰比较严重,则建议采用更有效的前处理方法,就是固相萃取方法。固相萃取(Solid Phase Extraction, 简称SPE)是一种包括液相和固相的萃取过程,是通过填料的键合官能基团和待分离化合物之间的作用力,将目标化合物与样品基质分离,以达到除去杂质和富集目标组分的目的,固相萃取小柱的种类较多,用于生物样品净化前处理的固相萃取填料主要有以下几种:
■固相萃取填料类型和结构
■根据化合物类型选择对应的固相萃取填料
■通用操作方法
固相萃取产品订购信息
【EVOLUTE EXPRESS系列】
■聚合物基质反相填料—EVOLUTE EXPRESS ABN
96-Well PlatePN. | Description | Qty. |
---|
600-0010-PX01 | EVOLUTE Express ABN 10mg Plate | 1 |
600-0030-PX01 | EVOLUTE Express ABN 30mg Plate | 1 |
Tabless SPE ColumnPN. | Description | Qty. |
---|
600-0001-AXG | EVOLUTE Express ABN 10mg/1 mL | 100 |
600-0003-AXG | EVOLUTE Express ABN 30mg/1 mL | 100 |
610-0006-BXG | EVOLUTE Express ABN 60mg/3 mL | 50 |
610-0010-BXG | EVOLUTE Express ABN 100mg/3 mL | 50 |
610-0015-CXG | EVOLUTE Express ABN 150mg/6 mL | 30 |
610-0050-CXG | EVOLUTE Express ABN 500mg/6 mL | 30 |
■混合型强阳离子交换填料—EVOLUTE EXPRESS CX
96-Well PlatePN. | Description | Qty. |
---|
601-0010-PX01 | EVOLUTE Express CX 10mg Plate | 1 |
601-0030-PX01 | EVOLUTE Express CX 30mg Plate | 1 |
Tabless SPE ColumnPN. | Description | Qty. |
---|
601-0001-AXG | EVOLUTE Express CX 10mg/1 mL | 100 |
601-0003-AXG | EVOLUTE Express CX 30mg/1 mL | 100 |
611-0006-BXG | EVOLUTE Express CX 60mg/3 mL | 50 |
611-0010-BXG | EVOLUTE Express CX 100mg/3 mL | 50 |
611-0015-CXG | EVOLUTE Express CX 150mg/6 mL | 30 |
611-0050-CXG | EVOLUTE Express CX 500mg/6 mL | 30 |
■混合型强阴离子交换填料—EVOLUTE EXPRESS AX
96-Well PlatePN. | Description | Qty. |
---|
601-0010-PX01 | EVOLUTE Express CX 10mg Plate | 1 |
601-0030-PX01 | EVOLUTE Express CX 30mg Plate | 1 |
Tabless SPE ColumnPN. | Description | Qty. |
---|
601-0001-AXG | EVOLUTE Express CX 10mg/1 mL | 100 |
601-0003-AXG | EVOLUTE Express CX 30mg/1 mL | 100 |
611-0006-BXG | EVOLUTE Express CX 60mg/3 mL | 50 |
611-0010-BXG | EVOLUTE Express CX 100mg/3 mL | 50 |
611-0015-CXG | EVOLUTE Express CX 150mg/6 mL | 30 |
611-0050-CXG | EVOLUTE Express CX 500mg/6 mL | 30 |
■混合型强阴离子交换填料—EVOLUTE EXPRESS AX
96-Well PlatePN. | Description | Qty. |
---|
603-0010-PX01 | EVOLUTE Express AX 10mg Plate | 1 |
603-0030-PX01 | EVOLUTE Express AX 30mg Plate | 1 |
Tabless SPE ColumnPN. | Description | Qty. |
---|
603-0001-AXG | EVOLUTE Express AX 10mg/1 mL | 100 |
603-0003-AXG | EVOLUTE Express AX 30mg/1 mL | 100 |
613-0006-BXG | EVOLUTE Express AX 60mg/3 mL | 50 |
613-0010-BXG | EVOLUTE Express AX 100mg/3 mL | 50 |
613-0015-CXG | EVOLUTE Express AX 150mg/6 mL | 30 |
613-0050-CXG | EVOLUTE Express AX 500mg/6 mL | 30 |
■混合型弱阳离子交换填料—EVOLUTE EXPRESS WCX
96-Well PlatePN. | Description | Qty. |
---|
602-0010-PX01 | EVOLUTE Express WCX 10mg Plate | 1 |
602-0030-PX01 | EVOLUTE Express WCX 30mg Plate | 1 |
Tabless SPE ColumnPN. | Description | Qty. |
---|
602-0001-AXG | EVOLUTE Express WCX 10mg/1 mL | 100 |
602-0003-AXG | EVOLUTE Express WCX 30mg/1 mL | 100 |
612-0006-BXG | EVOLUTE Express WCX 60mg/3 mL | 50 |
612-0010-BXG | EVOLUTE Express WCX 100mg/3 mL | 50 |
612-0015-CXG | EVOLUTE Express WCX 150mg/6 mL | 30 |
612-0050-CXG | EVOLUTE Express WCX 500mg/6 mL | 30 |
■混合型弱阴离子交换填料—EVOLUTE EXPRESS WAX
96-Well PlatePN. | Description | Qty. |
---|
604-0010-PX01 | EVOLUTE Express WAX 10mg Plate | 1 |
604-0030-PX01 | EVOLUTE Express WAX 30mg Plate | 1 |
Tabless SPE ColumnPN. | Description | Qty. |
---|
604-0001-AXG | EVOLUTE Express WAX 10mg/1 mL | 100 |
604-0003-AXG | EVOLUTE Express WAX 30mg/1 mL | 100 |
614-0006-BXG | EVOLUTE Express WAX 60mg/3 mL | 50 |
614-0010-BXG | EVOLUTE Express WAX 100mg/3 mL | 50 |
614-0015-CXG | EVOLUTE Express WAX 150mg/6 mL | 30 |
614-0050-CXG | EVOLUTE Express WAX 500mg/6 mL | 30 |
■EVOLUTE EXPRESS 系列方法开发板
EVOLUTE EXPRESS 系列方法开发板包含四种混合模式的填料, EVOLUTE EXPRESS CX,WCX, AX和WAXPN. | Description | Qty. |
---|
650-0010-PX01 | EVOLUTE Express Sorbent Selection Plate 10mg | 1 |
650-0030-PX01 | EVOLUTE Express Sorbent Selection Plate 30mg | 1 |
【Panthera 和Agility系列 】
■聚合物基质反相填料-Panthera
96-Well PlatePN. | Description | Qty. |
---|
OMNPA6030 | Panthera Deluxe,Polymer DVB 96-well plate,30mg/1mL | 1 |
OC21PA6030 | Panthera Deluxe,Polymer DVB 96-well plate,30mg/2mL | 1 |
SPE ColumnsPN. | Description | Qty. |
---|
SYPA6030-01RL | Panthera Deluxe, 30mg/1cc rimless long luer cartridges | 1 |
■混合型强阳离子交换填料—Agility SCX
96-Well PlatePN. | Description | Qty. |
---|
OMNAG2030 | Agility SCX, Polymer DVB 96-well plate, 30 mg/1 mL | 1 |
OC21AG2030 | Agility SCX, Polymer DVB 96-well plate, 30 mg/2 mL | 1 |
OMNAD2030 | Agility Deluxe SCX, Polymer DVB 96-well plate, 30 mg/1 mL | 1 |
OC21AD2030 | Agility Deluxe SCX, Polymer DVB 96-well plate, 30 mg/2 mL | 1 |
SPE ColumnsPN. | Description | Qty. |
---|
SYAG2025-01RL | Agility SCX, 30mg/1cc rimless long luer cartridges | 100 |
SYAD2030-01RL | Agility Deluxe SCX, 30mg/1cc rimless long luer cartridges | 100 |
■混合型强阴离子交换填料—Agility SAX
96-Well PlatePN. | Description | Qty. |
---|
OMNAG5030 | Agility SAX, Polymer DVB 96-well plate, 30 mg/1 mL | 1 |
OC21AG5030 | Agility SAX, Polymer DVB 96-well plate, 30 mg/2 mL | 1 |
SPE ColumnsPN. | Description | Qty. |
---|
SYAG5025-01RL | Agility SAX, 30mg/1cc rimless long luer cartridges | 100 |
■混合型弱阳离子交换填料—Agility WCX
96-Well PlatePN. | Description | Qty. |
---|
OMNAG8030 | Agility WCX, Polymer DVB 96-well plate, 30 mg/1 mL | 1 |
OC21AG8030 | Agility WCX, Polymer DVB 96-well plate, 30 mg/2 mL | 1 |
SPE ColumnsPN. | Description | Qty. |
---|
SYAG8025-01RL | Agility WCX, 30mg/1cc rimless long luer cartridges | 100 |
■混合型弱阴离子交换填料—Agility WAX
96-Well PlatePN. | Description | Qty. |
---|
OMNAD7030 | Agility Deluxe WAX, Polymer DVB 96-well plate, 30 mg/1 mL | 1 |
OC21AD7030 | Agility Deluxe WAX, Polymer DVB 96-well plate, 30 mg/2 mL | 1 |
SPE ColumnsPN. | Description | Qty. |
---|
SYAD7030-01RL | Agility Deluxe WAX, 30mg/1cc rimless long luer cartridges | 100 |
■96孔板底座——可支持 SPE Columns
PN. | Description | Qty. |
---|
ORO1MLBLK | 1mL 96-Well holder block | 100 |