研域生物 品牌
经销商厂商性质
上海市所在地
本产品仅用于科研,不用于临床
MOC2细胞系冻存或复苏、传代或原代、进口及国产提供,公司供应的细胞生长健康,活性优良,现公司能提供给客户的细胞株及细胞系达三千多种,涵盖细胞所有研究领域,国内外科研单位,我们供货。质量保证,可定制包装规格,发货快速,同各个相关科研机构建立了良好的合作关系,欢迎您、订购优势的产品!
FisherScientific:
T150Flasks : 07-200-64
T75 Flasks : 10-126-37
Cryovials : 03-374-059
45um filters: : 09-754-21
05% Trypsin : sh30236.01
25% Trypsin : sh30042.01
Indolent Lines – MOC1, 22
Aggressive Lines – MOC2
Thawing cell lines
1. Add 21ml IMDM MOC line media to a T150 before thawing (or 10ml to a T75 if wanting to thaw into aT75)
2. Remove cryovial from liquid nitrogen, spray vial with 70% alcohol to clean it.
3. Hold bottom-half of cryovial in 37Cwaterbath (without letting lid touch water, to avoid contamination) until there is a small chunk of ice left floating.
4. Spray cryovial again with ETOH and place in hood. Pipette 1ml of media to the 1ml of cells and add these 2ml to the T150 that already contains media (to make 22ml total for one T150).
5. Take some media already in T150 flask and rinse the cryovialand plate this to ensure you have all the residual cells left in the cryovial.
MOC2细胞系保存注意事项:
低温、避光、干燥阴凉处封闭贮存,严禁与有毒、有害物品混放、混运。本品为非危险品,可按一般化学品运输,轻搬动轻放,防止日晒、雨淋。