floating aggregates of round cells with some attached cells
Organism:
Homo sapiens （human）
Morphology:
epithelial
Source:
Organ: cecum Disease: colorectal adenocarcinoma
Cellular Products:
carcinoembryonic antigen （CEA）, 1709 ng/ml per 10 exp6 cells per 10 days
Permits/Forms:
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NCI-H508 人结肠直肠腺癌
Tumorigenic:
Yes
Antigen Expression:
Blood type A; Rh+; CA19-9 antigen
DNA Profile （STR）:
Amelogenin: X
CSF1PO: 11,12
D13S317: 8,12
D16S539: 12
D5S818: 12
D7S820: 8,12
THO1: 9,9.3
TPOX: 8,11
vWA: 15,16
Cytogenetic Analysis:
modal number = 102; range = 71 to 131; most cells have DM's
Isoenzymes:
AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1
Age:
55 years
Gender:
male
Ethnicity:
Caucasian
Comments:
This line was derived from a metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil.
The cells contain Dopa decarboxylase, CA19-9 antigen and CEA but do not express the TAG-72 antigen.
Propagation:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:
S
Preservation:
Culture medium, 95%; DMSO, 5%
References:
23066: Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249
