简单介绍新一代无标记酶标仪 BYOSENS LYTE96*台便携式无标记酶标仪  该BYOSENS LYTE96*台便携式无标记酶标仪是*款便携式无标记酶标仪。根据康宁Epic系统它被设计为读出96孔板，并进行了广泛的细胞化验。结合无线连接和集成电池结合，lyte96的紧凑的结构使得它*移动并易于在液体处理系统集成。 The next generation label-free reader BYOSENS LYTE96 THE

*台便携式无标记酶标仪,BYOSENS LYTE96 PORTABLE LABEL-FREEMICROPLATE READER的详细介绍

BYOSENS LYTE96*台便携式无标记酶标仪（便携微孔板检测器）

LYTE96便携式无标记酶标仪（便携微孔板检测器）是基于康宁Epic系统设计的，可进行一系列细胞内试验的96孔微孔板读出设备。lyte96将无线连接和集成电池结合放置到一个紧凑的结构中，使得它方便移动和易于整合进液体处理系统。主要是对系列广泛的生物反应进行检测，如信号转导、细胞凋亡、细胞毒素，贴壁、增殖和扩散等。

lyte96无标记便携生物传感器的工作原理是基于折射波导光栅光学生物传感器。传感器结构由一个三层系统：玻璃基板、薄膜光波导薄膜与光栅结构，和细胞/生物分子层。当宽谱带光照射时，生物传感器反映光的特定波长是接近传感器表面折射率的灵敏函数。通过 Epic系统测量细胞内的粘合物事件或细胞内蛋白质运动引起反射光的波长偏移。形成一系列波长偏移、波长、强度、时间之间的函数来进行分析。

lyte96无标记便携生物传感器的优势：

移动性： lyte96创新设计之处是给使用者带来了极大的灵活性。紧凑的结构结合了无线连接和集成的电池使lyte96方便移动。这使得它对于研究人员和开发人员来说成为一个*的分析工具。

易用性： lyte96简化了研发实验室中的过程。实验开始时不需要复杂的预置，直观辅助的软件保证了高水平的易用性。由于技术体系，lyte96几乎是免费维护。

数据分析：根据已建立的康宁Epic系统，高敏性的lyte96可进行宽光谱的细胞内试验，从开始试验到几天的时间都可以提供实时数据以便研究。

图1. 莱特96无标记便携生物传感器

图2.测量原理示意图

例1：在增殖试验中，用lyte96实时监测细胞数量，发现细胞数目和传感器表面的质量是成正比的。微孔板和lyte96放置在加湿的培养箱内通过蓝牙无线连接电脑。经典增殖试验中，A431细胞加入到孔中，记录37?C的细胞生长。

例2：动态质量再分配（DMR）的测定

像许多其他的信号检测，GPCR测定动态质量再分配过程中（DMR）是由lyte96无标记传感器测定的。和A431细胞缓激肽试验一样，这个试验是在室温下进行。得到的EC50为0.45 nm，这类似于从文献的结果。

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