flexcell SpillGuard®培养板， SpillGuard® cul

flexcell SpillGuard®培养板， SpillGuard® cul

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世联博研（北京）科技有限公司

世联博研（北京）科技有限公司

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产品简介

BioFlex双向拉应力培养板, Uniflex单向拉应力培养板 、TissueTrain三维细胞组织培养板等系列细胞培养一起使用,
培养板类型、包被表面材料丰富：Amino, Collagen （Type I or IV）, Elastin, ProNectin （R GD）, Laminin （YIGSR）.表面涂层丰富的
包被材料， 您可以跟根据不同细胞组织可以灵活选择不同包被材料表面

详细介绍

 

BioFlex双向拉应力培养板, Uniflex单向拉应力培养板 、TissueTrain三维细胞组织培养板等系列细胞培养一起使用,
培养板类型、包被表面材料丰富：Amino, Collagen (Type I or IV), Elastin, ProNectin (R GD), Laminin (YIGSR).表面涂层丰富的
包被材料， 您可以跟根据不同细胞组织可以灵活选择不同包被材料表面 （包被材料选择参考）。

该应力加载系统配套培养板zui大伸展率
Culture Plate for tension systemMax Achievable Strain
BioFLEX®双向应力细胞培养板(BioFLEX®CULTURE PLATES)21.80%
Tissue Train三维细胞组织培养板(Tissue Train culture plates)20.80%
UniFlex™单向应力细胞培养板(UniFlex™Culture Plates)12.20%
24孔高通量BIOFLEX®培养板培养板（HT BIOFLEX®CULTURE PLATES）15.00%
No Loading Stations33%

1、BioFLEX®双向应力细胞培养板(BioFLEX®CULTURE PLATES)
双向应力细胞培养板,BioFLEX CULTURE PLATES

BIOFLEX®CULTURE PLATES（BIOFLEX®培养板培养板）

编号产品

产品名称

BF-3001U-Case（每箱40块）BF-3001E-Each(每块)BioFlex Culture Plate-Untreated（6孔表面未处理的BioFLEX®双向应力细胞培养板）
BF-3001A-Case（每箱40块）BF-3001A-Each(每块)BioFlex Culture Plate-Amino （6孔氨基BioFLEX®双向应力细胞培养板）
BF-3001C-Case（每箱40块）BF-3001C-Each(每块)BioFlex Culture Plate-Collagen Type I （6孔胶原I 型BioFLEX®双向应力细胞培养板）
BF-3001C(IV)-Case（每箱40块）BF-3001C(IV)-Each(每块)BioFlex Culture Plate-Collagen Type IV （6孔胶原IV型BioFLEX®双向应力细胞培养板）
BF-3001E-Case（每箱40块）BF-3001E-Each(每块)BioFlex Culture Plate-Elastin （6孔弹力BioFLEX®双向应力细胞培养板）
BF-3001P-Case（每箱40块）BF-3001P-Each(每块)BioFlex Culture Plate-ProNectin （6孔 ProNectin BioFLEX®双向应力细胞培养板）
BF-3001L-Case（每箱40块）BF-3001L-Each(每块)BioFlex Culture Plate-Laminin （6孔层粘连蛋白BioFLEX®双向应力细胞培养板）

2、UniFlex™单向应力细胞培养板(UniFlex™Culture Plates)

flexcell,UniFlex（flexcell双向应力培养板）

 

BioFlex® Culture Plate 
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Flexible-bottomed culture plate that works with the Flexcell® FX-5000™ Tension Systemto provide a mechanical load regimen to cells in monolayer (see Fig. 1 below).
  • 6-well culture plate with total growth surface area of 57.75 cm2.
  • Rubber membranes are optically clear for direct viewing of cells with an inverted or upright microscope.
  • Covalently bound matrix surfaces: Amino, Collagen (Type I or IV), Elastin, Pronectin® (RGD), and Laminin (YIGSR). Learn more in Tech Report 106: Matrix Bonded Growth Surfaces. Growing Cells in a More Natural Matrix Environment.
  • Available individually or by the case of 40* plates.
*5% Savings (based on buying 40 plates at the individual price)


Applications of BioFlex® Culture Plates
  • Mechanical Load - use with the FX-5000™ Tension System to research the effects of equibiaxial tensile strain on cells in monolayer culture.
  • Softer Substrate - provides a softer substrate for cell growth (when compared to standard plastic culture dishes) which better mimics in situ conditions for some cell types like trabecular meshwork cells (see Aga et al. Invest Ophthalmol Vis Sci 55(9):5497-509, 2014).
  • Matrix Coated Surface - culture plate surfaces are coated with various matrix proteins, such as type I collagen and laminin, to better simulate in vivo conditions (see Tech Report 106: Matrix Bonded Growth Surfaces. Growing Cells in a More Natural Matrix Environment).
  • Traumatic Injury - can be used in studies looking at traumatic injury in astrocytes and neurons, where the injury is produced with a Cell Injury Controller that controls a burst of gas which deforms the BioFlex® membrane creating large biaxial strains (see Ellis et al. J Neurotrauma 12 325–339, 1995; Ahmed et al. J Neurochem 74(5):1951-1960, 2000; Augustine et al. Neuroscience 274:1-10, 2014).
Read more about Applying Mechanical Load to Cells in Monolayer Culture
 
Equibiaxial Strain

Figure 1: Equibiaxial strain application (with a FX-5000™ Tension System) to cells plated in the well of a BioFlex® culture plate.

Relevant Tech Reports & Other Information
  • 101: Loading Stations™. Quantification of Strain on the Membrane Surface
  • 106: Matrix Bonded Growth Surfaces. Growing Cells in a More Natural Matrix Environment
  • 202: Viewing Cells on Flexcell®'s Culture Plates. Light and Electron Microscope Techniques and Immunofluorescent Imaging 
     
  • BioFlex® Culture Plates Product Information
  • 6-Well Equibiaxial Loading Stations™ Product Information
  • FlexStop™ Product Information
  • Flexcell® Transwell Holder Product Information 
     
  • Culture Plate & Loading Station™ User's Manual
  • Flexcell® FX-5000™ Tension System User Manual
  •  

 

 

The History of Flexcell®
In 1978, Albert J. Banes, Ph.D., an Assistant Professor in the Surgery Department at the University of North Carolina at Chapel Hill, received his first small grant for devising a method to apply regulated strain to cultured cells. He built prototypes of the current Flexcell® Tension System in the shop using only a timer, a simple solenoid valve to control pressure in on/off modes, and a needle valve to regulate the magnitude of the pressure. He built a baseplate with vacuum channels in Plexiglas and used natural gum rubber as the seal between the culture plate bottom and the vacuum chamber. Dr. Banes polymerized his own rubber membranes from medical grade Dow silastic components. A cast material that eventually proved satisfactory for the membrane in a 6-well, 35 mm diameter culture plate that had the well bottoms drilled out to receive the cast membranes. A Commodore Vic 20 was the first commercial microprocessor used to control the valves. The software program and a controller board were designed by Dr. Banes and a well grounded electronics engineer, Olivier Monbureau. A Commodore 64 was the next generation computer used, followed by a Tandy computer, then IBM clones. Today, Flexcell® develops its own software and builds custom computers and controller boards and assembles them at its plant in Burlington, NC.

Dr. Banes' inlectual property was awarded the first patent in the field of Cytomechanics. He believed that this technology could become important scientifically as well as medically if a market driver could be found. In his first patent describing the technology, he included the thought that cyclically stretching cells and tissues could lead to adaptation of cells to a mechanically active environment such as dermal fibroblasts cultured in vitro in a skin construct. Along with his father in Pennsylvania, G.B. Banes, J.D., they created Flexcell® Inc. The company's mission was to commercialize the technology to make it available to all scientists as well as find medically relevant uses. His first full publication (Journal of Cell Science, 1985) in the area demonstrated the utility of the system and that cyclic strain altered the expression of cytoplasmic filament proteins such as actin and tubulin in tendon cells.

Continued development of the technologies behind the Flexcell® Tension and Compression Systems has taken much of Dr. Banes' time. Dr. Banes became the first university professor to be permitted to retain his academic professorship and be president of a company (Flexcell® International Corporation) by a decision of the Committee on Industrial Faculty Relations at the University of North Carolina at Chapel Hill.

Dr. Banes holds three patents on the basic cell stretching technology and several more on other technologies including a DNA transfection technique using flexation of cells and a spill resistant culture plate called the SpillGuard® culture plate. He continues to develop new products for cell culture and cell stretching as well as submit patents assigned to Flexcell® International Corporation.
 

 


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