- quickzyme是欧洲著名的EILISA 试剂盒提供商,为很多大的试剂公司OEM试剂盒,其产品高质量和高灵敏性及稳定性得到各大实验室及制药公司认可,其Granzyme B ELISA KIT 早已经被世界各大实验室使用,高品质的质量得到众多客户的选择
- bace1(β-site app cleaving enzyme),即β-分泌酶,是淀粉样前体蛋白(amyloid precursor protein,app)产生aβ的关键酶。bace1属于ⅰ型跨膜蛋白,由501个氨基酸组成,其中46~460位氨基酸为功能活性区域。bace1通过水解app670met-671asp之间的肽键,生成670个氨基酸的可溶性n末端和包含完整aβ区的app跨膜c端。后者经γ分泌酶进一步酶切形成aβ。zui近的研究表明,bace1基因敲除小鼠脑内的aβ水平可降低90%,且其生理表型基本正常,显示了bace1抑制剂在ad治疗中的广阔前景[1]。通过酵母或昆虫表达系统表达活性bace1在zui近已见报道[2,3]。然而,由于上述系统表达的蛋白量低,且纯化流程复杂,不适合bace1抑制剂的大规模筛选。而bace1的原核表达可克服以上缺陷,为筛选特异性抑制剂及研究bace1结构与功能关系提供大量材料,为zui终从根本上治疗ad带来希望。
- Assay specifications
- Reagent set for five 96-well plates
- Measures active human Granzyme B
- Samples: conditioned cell culture media, cell extracts, body fluids (plasma, wound fluid ), tissue extracts, and (purified) enzyme preparations
- Quantitative
- Range/sensitivity: 0.01 ng/ml (4 h incubation)
- Ease-of-use: Equivalent to ELISA
- Overnight protocol
- Store at 20°C for long term storage
- Reagent set contains antibody, standard, detection enzyme and substrate
This kit is developed by QuickZyme BioSciences and is available via Sigma Aldrich.