B27细胞培养添加剂invitrogen/Gibco（货号17504-044）说明书

描述

B-27® 添加剂是无血清添加剂，用于海马神经元和其他中枢神经系统（CNS）神经元的生长和保持其短期或长期活性。B-27® 添加剂是50X 的液体，可以与Neurobasal® 培养基或Neurobasal®-A 培养基组合使用，用于神经细胞培养而不需要再添加饲养层细胞。

B-27® 添加剂适和应用于大多数神经细胞培养。对于特别的应用，我们提供 其他配方的B-27® 添加剂.

质量控制检测 
B-27® 添加剂 进行了18天妊娠的原代大鼠（Sprague Dawley） 的胚胎海马神经元生长检测。 通过了细菌和真菌的检测. B-27® 也做了1X 浓度的内毒素水平测试。 

仅供研究使用。除非另有说明，不得用于任何动物或人类治疗或诊断。

详细说明

B27细胞培养添加剂invitrogen/Gibco（货号17504-044）说明书 常用规格

引文和参考文献 （17）

• D2 receptors inhibit the secretory process downstream from calcium influx in dopaminergic neurons: implication of K+ channels.

作者

Congar P; Bergevin A; Trudeau LE

杂志

J Neurophysiol （2002） 87:1046-1102

产品用途

Using a culture system in which isolated DAergic neurons establish self-innervating synapses （"autapses"） that release both DA and glutamate, we studied the mechanism by which presynaptic D2 receptors inhibit glutamate-mediated excitatory postsynaptic currents （EPSCs）. 

ID:

PN64425

• Decreased synaptic vesicle recycling efficiency and cognitive deficits in amphiphysin 1 knockout mice.

作者

Di Paolo Gilbert; Sankaranarayanan Sethuraman; Wenk Markus R; Daniell Laurie; Perucco Ezio; Caldarone Barbara J; Flavell Richard; Picciotto Marina R; Ryan Timothy A; Cremona Ottavio; De Camilli Pietro;

杂志

Neuron （2002） 33:789-804

产品用途

Primary cultures of cortical neurons were prepared. Cells were maintained up to 3 weeks in Neurobasal/B27 medium at 37°C in a 5% CO2 humidified incubator. 

ID:

PN47418

• Blocking HES1 expression initiates GABAergic differentiation and induces the expression of p21（CIP1/WAF1） in human neural stem cells.

作者

Kabos Peter; Kabosova Andrea; Neuman Toomas;

杂志

J Biol Chem （2002） 277:8763-8769

产品用途

Resected human fetal brain tissue was placed into ice cold DMEM/F-12 with penicillin/streptomycin for further dissection. The tissue was cut into small pieces and trypsinized （0.02 mg/ml trypsin in versene （Invitrogen） at 37 °C for 10 min. Cell suspension was centrifuged at 400 rpm for 5 min, the pellet was washed once with DMEM/F-12 and plated at a density of 5000-10,000 viable cells/ml in the growth medium DMEM/F-12, B27 supplement （Invitrogen）. Cells were washed with PBS （Invitrogen）  

ID:

PN63471

• Long-term culture of mouse cortical neurons as a model for neuronal development, aging, and death.

作者

Lesuisse Christian; Martin Lee J;

杂志

J Neurobiol （2002） 51:9-23

产品用途

A long-term cell culture system was used to study maturation, aging, and death of cortical neurons. 

ID:

PN60461

• An improved method for the expression of TRH in serum-supplemented primary cultures of fetal hypothalamic cells.

作者

Joseph-Bravo Patricia; Pérez-Martínez Leonor; Lezama Leticia; Morales-Chapa Claudia; Charli Jean Louis;

杂志

Brain Res Brain Res Protoc （2002） 9:93-104

产品用途

Primary cultures of dispersed cells from fetal nervous tissue are extensively used for studying multiple neuronal properties 

ID:

PN62028

更多..

回到页首

按细胞系排列的引文

慧颖生物主营：生化试剂、抗体、重组蛋白、细胞生物、分子生物、蛋白研究、微生物、Elisa试剂盒、耗材、标准品、 标准溶液……

abcam、ABI、Axygen、corning、CST、Gibco、Hyclone、invitrogen、merck、Millipore、Qiagen、R&D、Roche、santa、sigma、Thermo等进口品牌……

以下为相关本公司常备现货产品

*部分 invitrogen 原装产品

第二部分 HyClone胎牛血清

第二部分  Gibco胎牛血清