CRL-10609 MMQ 大鼠垂体瘤细胞 Designations:MMQ Depositors: University of Virginia Patent Foundation Biosafety Level:1 Shipped:frozen Medium & Serum:See Propagation
详细介绍
CRL-10609 MMQ 大鼠垂体瘤细胞
ATCC® Number:
CRL-10609™
Price:
CRL-10609 MMQ 大鼠垂体瘤细胞
Designations:
MMQ
Depositors:
University of Virginia Patent Foundation
Biosafety Level:
1
Shipped:
frozen
Medium & Serum:
See Propagation
Growth Properties:
suspension
Organism:
Rattus norvegicus （rat）
Morphology:
epithelial
Source:
Organ: pituitary Disease: tumor
Cellular Products:
prolactin
Permits/Forms:
In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Receptors:
dopamine
Tumorigenic:
No
Age:
5 days
Comments:
The cells secrete prolactin and express functional dopamine receptors [U.S. Patent 5,510,260].
Propagation:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium:
fetal bovine serum to a final concentration of 2.5%
horse serum to a final concentration of 15% Atmosphere: air, 95%; carbon dioxide （CO2）, 5% Temperature: 37.0°C DGrowth Conditions: The line grows as clumps of cells in suspension.
Subculturing:
Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10（5） viable cells/ml and maintain between 1 X 10（5） and 1 X 10（6） cells/ml. Medium Renewal: Every 2 days
Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2004 recommended serum:ATCC 30-2020 recommended serum:ATCC 30-2040 Cell culture tested DMSO:ATCC 4-X
References:
22090: MacLeod RM, et al. Prolactin secreting cell line and method of obtaining the same. US Patent 5,510,260 dated Apr 23 1996
