猪繁殖与呼吸综合征病毒抗体检测试剂盒

名称和用途

Prionics 蓝耳病 猪繁殖与呼吸综合征抗体检测试剂盒是用于检测猪血清和血浆中的猪繁殖与呼吸综合征病毒(PRRSV)抗体的酶联免疫检测试剂盒。

概述

猪繁殖与呼吸综合征病毒（PRRSV）是一种动脉炎病毒，在80 年代后期*在欧洲和美国发现，两种毒株具有不同的抗原性。该病毒引起繁殖问题（ 流产，产弱仔，死胎， 母猪死亡）、呼吸系统疾病（肺炎，呼吸困难，行动迟缓，死亡）和轻度神经症状1。猪繁殖与呼吸综合征引起严重的经济损失，被认为是影响养猪业zui重要的疾病之一。可以通过便捷的检测血清或血浆中的抗体评估猪只是否感染PRRSV（无论是自然感染或人工免疫疫苗）。需要注意的是这种检测抗体的免疫学方法需要对不同的PRRS 毒株的检测都很敏感。

此试剂盒仅供体外检测使用。

原理

Prionics试剂盒通过*的抗体设计，能同时检测美洲株和欧洲株不同血清型的蓝耳病毒。将重组的ORF7蛋白 （美洲株和欧洲株）包被在微孔滴定板上，被测样品在包被孔中孵育时，针对PRRSV的特异性抗体与包被的抗原形成抗原抗体复合物，洗去包被孔中没有结合的成分后，加入辣根过氧化物酶（HRPO）标记的抗猪抗体，它与孔中的猪抗体结合，在试验的zui后步骤，洗去没有结合的酶标抗体，再加入酶底物和显色剂TMB，颜色反应的深浅由样品中存在的抗PRRSV 特异性抗体的量决定。

The PrioCHECK? PRRSV Antibody ELISA is specially designed for the detection of both the American and the European genotypes of the PRRS virus in serum samples. Plates are coated with ORF 7 protein (common to both genotypes) ensuring the detection of antibodies to both virus types with high sensitivity and specificity. The PrioCHECK? PRRSV Antibody ELISA shows a high agreement with the most frequently used ELISA in the field and is therefore the most powerful alternative on the PRRS test market.

Test procedure
The PrioCHECK? PRRSV Antibody ELISA is a diagnostic kit based on ELISA technology. It follows a simple four step protocol consisting of sample preparation, sample incubation, conjugate incubation, and detection. Easy handling makes the test convenient and fast: 90 samples can be analyzed within 150 minutes. Serum samples are incubated in an ELISA plate coated with the ORF 7 protein of PRRSV. Anti-PRRSV antibodies present in the sample bind to the antigen. Plates are then washed and an anti-swine antibody labeled with horse radish peroxidase (HRPO) is used to detect antibodies bound to the ORF 7 coated plate. Color development using tetramethyl-benzidine (TMB) substrate measured optically at a wavelength of 450 nm shows the presence of antibodies directed against PRRSV.