产品英文名称：CHO ELISA kit
产品中文名称：CHO 细胞 ELISA 试剂盒

CHO ELISA kit 的应用
本试剂盒是用于确定在CHO宿主细胞重组表达产品 中CHO蛋白污染存在。该试剂盒只用于研究和制造使用，不适合于人类或动物的诊断应用。

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CHO细胞 ELISA 试剂盒的英文描述
The antibodies used in the kit were generated against and affinity purified from a cell lysate of CHO cells grown in serum supplemented media. All three of the Cygnus CHO HCP kits recognize a qualitatively vast majority of the same HCPs. The potential exists that the quantitative value reported for a given sample type may differ between the kits, therefore we cannot predict which kit will be most suitable for your applications. Formal qualification must be performed on each different product, to determine which of the 3 kits are superior. For labs looking at CHO HCP contamination for the first time, we suggest evaluating our CHO HCP ELISA, 3G, Catalog # F550. If this method proves inadequate then we suggest evaluating CHO HCP kits, Catalog #CM015 and F015.

CHO ELISA kit 的使用步骤规则
The CHO Host Cell Protein assay is a two-site immunoenzymetric assay. Samples which may contain CHO proteins are reacted with an affinity purified alkaline phosphatase labeled antibody. This reaction either takes place in a test tube in a sequential assay mode or simultaneously in microtiter wells coated with an anti-CHO HCP capture antibody.The two-step sequential protocol or the one step simultaneous
protocol result in the formation of a sandwich complex of solid phase (capture) antibody-CHO protein-enzyme labeled antibody. The microtiter strips are then washed to remove any unbound reactants. The substrate p-nitrophenyl phosphate (PNPP) is then reacted. The amount of hydrolyzed substrate is read on a microtiter plate reader and will be directly proportional to the concentration of CHO proteins present.

CHO ELISA kit 的保存和稳定性
* All reagents should be stored at 2°C to 8°C for stability until the expiration date printed.
* The substrate reagent should not be used if its absorbance at 405nm is greater than 0.4.
* Reconstituted wash solution is stable until the expiration date of the kit.

CHO ELISA kit 的使用注意事项
*只用于研究或制造。
*在用本试剂盒的试剂浓度，其他没有被认为是有害的。
*本试剂盒只能由合格的技术人员使用。

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StemRD   MGro-500  MSC medium,chemically-defined,间充质干细胞培养基 500mL/瓶

LONZA   04-418Q    X-VIVO 15 无血清培养基 1L/瓶

Life Technology   087-0112DK    AIM-V® CTS™ 无血清细胞培养基（细胞治疗级） 1000mL/瓶

Pall   15950-017  Ultroser Serum Substitute 血清替代品 20mL/瓶

LONZA   12-725F   UltraCULTURE Serum-free Medium无血清培养基 500mL/瓶

BD 762165   PAXgene Blood RNA Tube RNA样本收集管 100管/盒

LONZA 50150   SeaKem® Gold Agarose 金琼脂糖粉 125g/瓶

LONZA CC-3162   EGM-2 BulletKit( CC-3156 & CC-4176 )套装 Kit/套

Nunc 159910   175cm2易用培养瓶,PS,已灭菌,瓶盖:过滤盖 30个/箱

LONZA LT07-318  MycoAlert® Mycoplasma Detection Kit支原体检测试剂盒 100Tests/盒

LONZA   LT07-418 MycoAlert® Mycoplasma Detection Kit支原体检测试剂盒 50Tests/盒

Lonza   50-650U   Kinetic-QCL 192 Test Kit内毒素检测试剂盒   192T/盒

Corning   431080    175cm⒉细胞培养瓶，Angled颈，透气盖 50个/箱

Corning   8388  125mL储液瓶 Storage Bottles,PS,45mm瓶颈，带盖，灭菌，独立包装 24个/箱

Corning   430659    2ml冻存管, 聚丙烯，外旋盖, 可自立圆底 500个/箱

Corning   3290     75cm2 CellBIND Surface培养瓶，已灭菌，透气盖 100个/箱

Corning   3292     175cm2 CellBIND Surface培养瓶，已灭菌，透气盖 50个/箱

BD-Falcon   352360   Cell Strainer, 100µm, Yellow 100um细胞滤网 50个/箱

BD Falcon 352070 50mL锥形离心管，高透明度聚丙烯,袋装 500支/箱

BD Falcon 352096 15mL锥形离心管，高透明度聚丙烯,袋装 500支/箱

BD BioCoat 354234   Matrigel Basement Membrane Matrix(标准型) 10mL/瓶

Cygnus   F550   CHO HCP ELISA Kit,3G宿主蛋白检测试剂盒 96T/盒

Cygnus   F410   E.coli HCP ELISA Kit 大肠杆菌 96T/盒

Nalgene  5100-0001 Cryo 1℃ Freezing Container梯度降温冻存盒 1个/箱

Nalgene    5026-0909   CryoBoxes 冻存盒 9X9 阵列，管瓶1.2/2.0mL   24个/箱

Nalgene   2250-0130 50L 可高温高压操作的细口大瓶（带手柄）   1个/箱

Nalgene   2319-0050    20L 可高温高压灭菌的细口大瓶（带放水口）    1个/箱

Hyclone  SH30084.03 FBS 优等澳洲胎牛血清 500mL/瓶

PeproTech   100-18B-500   Recombinant Human FGF-basic (154 a.a.)因子 500ug/支

Parafilm PM-996   Parafilm® M Laboratory Film 封口膜 12卷/箱