origen 现货热卖,欢迎咨询!!!
时间:2020-11-10 阅读:1390
ORIGEN 授权代理,种类齐全,现货供应,现双十一*,*销售,欢迎咨询!!!
背景知识:
细胞冻存是细胞保存的主要方法之一。利用冻存技术将细胞置于-196℃液氮中低温保存,可以使细胞暂时脱离生长状态而将其细胞特性保存起来,这样在需要的时候再复苏细胞用于实验。而且适度地保存一定量的细胞,可以防止因正在培养的细胞被污染或其他意外事件而使细胞丢种,起到了细胞保种的作用。除此之外,还可以利用细胞冻存的形式来购买、寄赠、交换和运送某些细胞。 细胞冻存时向培养基中加入保护剂--终浓度5%.15%的甘油或二甲基亚砜(DMSO),可使溶液冰点降低,加之在缓慢冻结条件下,细胞内水分透出,减少了冰晶形成,从而避免细胞损伤。 采用"慢冻快融"的方法能较好地保证细胞存活。标准冷冻速度开始为-1 到 -2℃/min,当温度低于-25℃时可加速,到-80℃之后可直接投入液氮内(-196℃)。
二甲基亚砜(DMSO)是一种含硫有机化合物,常温下为无色无味的透明液体,是一种吸湿性的可燃液体,具有高极性、高沸点、热稳定性好、非质子、与水混溶的特性,能溶于乙醇、丙醇、苯等大多数有机物,被誉为“万能溶剂”。二甲基亚砜是一种重要的渗透性细胞保护剂。在深低温(零下200度)保存细胞时冻存过程中,为防止细胞内液冰晶形成、渗透压改变、细胞结构紊乱等导致的损伤,有必要使用含有DMSO冷冻保护剂。DMSO能够快速穿透细胞膜进入细胞中,降低冰点,延缓冻存过程,同时提高细胞内离子浓度,减少细胞内冰晶的形成,从而减少细胞损伤。深低温时二甲基亚砜的细胞毒性受到抑制。复苏时动作要快,尽快洗掉二甲基亚砜,否则会造成对细胞严重的毒性。二甲基亚砜(DMSO)是目前的细胞冻存保护剂,但也是一种细胞毒性很大的化学试剂。研究结果表明,培养液中DMSO浓度为10%时,细胞生长抑制率近100%;1%浓度时抑制率为35%,即使是0.04%的浓度,DMSO对细胞的生长也有不利的影响。
右旋糖酐系蔗糖是经肠膜状明串珠菌-1226(Leuconostoc mesenteroides)发酵合成的一种高分子葡萄糖聚合物,右旋糖酐-40(Dextran-40)别称低分子右旋糖酐,右旋糖酐40的重均分子量(Mw)应为32000~42000。右旋糖酐-40可以维持渗透压和 PiH , 提供适宜细胞存活的介质环境。
OriGen公司研发的二甲亚砜(DMSO)在高级别DMSO的精制领域中处于地位,其细胞冻存级产品在市场上拥有较高的占有率,经过十多年的客户检验与使用,质量过硬。
ORIGEN 授权代理,种类齐全,现货供应,现双十一*,*销售,欢迎咨询!!!
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冻存袋 | 货号 | 名称 | 规格 |
OriGen | CS25F | Freezing Bags,vol-max,10-25mL冻存袋 | 24个/箱 |
OriGen | CS25N | Freezing Bags,vol-max,10-25mL冻存袋 | 24个/箱 |
OriGen | CS50 | Freezing Bags,vol-max,10-30mL冻存袋 | 24个/箱 |
OriGen | CS50N | Freezing Bags,vol-max,10-30mL冻存袋,N型管路 | |
OriGen | CS250 | Freezing Bags,vol-max,30-70mL冻存袋 | 24个/箱 |
OriGen | CS250S | Freezing Bags,vol-max,30-70mL冻存袋 | 12个/箱 |
OriGen | CS250N | Freezing Bags,vol-max,30-70mL冻存袋,N型管路 | 24个/箱 |
OriGen | CS500 | Freezing Bags,vol-max,55-100mL冻存袋 | 24个/箱 |
OriGen | CS750 | Freezing Bags,vol-max,80-190mL冻存袋 | 24个/箱 |
OriGen | CS1000N | Freezing Bags,vol-max,120-275mL冻存袋,N型管路 | 24个/箱 |
OriGen | CS2000N | Freezing Bags,vol-max,180-450mL冻存袋 | 24个/箱 |
OriGen | CS2000NP (厂家停产) | Freezing Bags,vol-max,180-450mL冻存袋 | 12个/箱 |
OriGen | CS30M9 | 5腔室Cryostore Multi-Chamber Bag | 12个/箱 |
OriGen | CS24M9 | 4腔室Cryostore Multi-Chamber Bag | 12个/箱 |
培养袋 | 货号 | 名称 | 规格 |
OriGen | PL07-2G | PermaLife Cell Culture Bags | 1个/包 |
OriGen | PL30-2G | PermaLife Cell Culture Bags | 1个/包 |
OriGen | PL70-2G | PermaLife Cell Culture Bags | 1个/包 |
OriGen | PL120-2G | PermaLife Cell Culture Bags | 1个/包 |
OriGen | PL240-2G | PermaLife Cell Culture Bags | 1个/包 |
OriGen | PL240K | PermaLife Cell Culture Bags,定制 | 1个/包 |
OriGen | PL325K | PermaLife Cell Culture Bags,定制 | 1个/包 |
OriGen | PL325-2G | PermaLife Cell Culture Bags | 1个/包 |
OriGen | PL500P-2G | PermaLife Cell Culture Bags(50个起订) | 1个/包 |
OriGen | PL750-2G | PermaLife Cell Culture Bags | 1个/包 |
OriGen | PL1000P-2G | PermaLife Cell Culture Bags | 1个/包 |
OriGen | PL2000P2-2G | PermaLife Cell Culture Bags(50个起订) | 1个/包 |
冻存液DMSO | 货号 | 名称 | 规格 |
OriGen | CP-10 | Vial, 10ml CryoPur : 100% DMSO | 12瓶/盒 |
OriGen | CP-50 | Vial, 50ml CryoPur : 100% DMSO | 6瓶/盒 |
OriGen | CP-70 | Vial, 70ml CryoPur : 100% DMSO | 6瓶/盒 |
OriGen | CP-70-Bag | Vial, 70ml CryoPur : 100% DMSO | 70ml/瓶 |
OriGen | CP-100 | Vial, 100ml CryoPur : 100% DMSO | 6瓶/盒 |
OriGen | SP-10 | 10ml in 20ml syringe with stopcock and SCD tubing | 1 unit |
OriGen | SP-20 | 20ml in 20ml syringe with stopcock and SCD tubing | 1 unit |
OriGen | SP-30 | 30ml in 20ml syringe with stopcock and SCD tubing | 1 unit |
OriGen | SP-50 | 50ml in 20ml syringe with stopcock and SCD tubing | 1 unit |
混合液 | 货号 | 名称 | 规格 |
OriGen | CD-07 | CryoPur-D™ : 55% DMSO, 5% Dextran-40 w/v, remainder water for injection | |
OriGen | CD-35 | CryoPur-D™ : 55% DMSO, 5% Dextran-40w/v, remainder water for injection | |
OriGen | CD-50 | CryoPur-D™ : 55% DMSO, 5% Dextran-40 w/v, remainder water for injection | 6瓶/盒 |
OriGen | CD-50-Bag | CryoPur-D™ : 55% DMSO, 5% Dextran-40 w/v, remainder water for injection | 50ml/瓶 |
OriGen | CD-100-Bag | CryoPur-D™ : 55% DMSO, 5% Dextran-40 w/v, remainder water for injection | 100ml/瓶 |
OriGen | SD-07 | CryoPur-D™ : 55% DMSO, 5% Dextran-40w/v, remainder water for injection,带注射器 | |
OriGen | SD-10 | CryoPur-D™ : 55% DMSO, 5% Dextran-40 w/v, remainder water for injection,带注射器 | |
OriGen | SDS-05A-B | 5ml 55% DMSO, 5% Dextran 40 w/v, remainder 0.9% saline + 2ml air in 20ml syringe with stopcock and SCD tubing | |
OriGen | SDS-15A | 15ml 55% DMSO, 5% Dextran 40 w/v, remainder 0.9% saline in 20ml syringe with stopcock and SCD tubing | |
外袋 | 货号 | 名称 | 规格 |
OriGen | OW0419 | 10个/包 | |
OriGen | OW0909 | O-Wrap Overwrap Bags 外袋(适合CS25) | 10个/包 |
OriGen | OW0918-S | O-Wrap Overwrap Bags 外袋(适合CS25,CS50,PL07) | 10个/包 |
OriGen | OW0918* | O-Wrap Overwrap Bags 外袋(适合CS25,CS50,PL07) | 10个/包 |
OriGen | OW0918-100S | O-Wrap Overwrap Bags 外袋(适合CS25,CS50,PL07) | 100个/箱 |
OriGen | OW1430 | O-Wrap Overwrap Bags 外袋(适合CS250,CS750,PL30,PL240) | 10个/包 |
OriGen | OW1936 | O-Wrap Overwrap Bags 外袋(适合CS1000) | 10个/包 |
OriGen | OW1950 | O-Wrap Overwrap Bags 外袋 | 10个/包 |
OriGen | OW2436 | O-Wrap Overwrap Bags 外袋(适合CS2000N) | 10个/包 |
*备注:OW0918*是由100个每箱的大包装分装成10个每包的小包装 | |||
热合仪 | 货号 | 名称 | 规格 |
OriGen | CR6 | Biosealer CR 6 热合仪 | 1台/箱 |
OriGen | SZ1823 | SteriZip Overpouch(CS25, CS50 ) | 10个/包 |
OriGen | SZ2533 | SteriZip Overpouch(CS250, CS500, CS750 ) | 10个/包 |
OriGen | SZ3038 | SteriZip Overpouch(CS1000, CS2000) | 10个/包 |
OriGen | SZ2533-2M | SteriZip Overpouch,2.0毫米 | |
OriGen | SZ2533P-1 | SteriZip Overpouch,独立包装 | 50个/包 |
OriGen | SZ2533P-3 | SteriZip Overpouch,3个/小包 | 45个/包 |
配件 | 货号 | 名称 | 规格 |
OriGen | S-F10 | One spike to one female luer with 10cm of tubing and pinch clamp, 100% DMSO Resistant | 50个/箱 |
OriGen | RF-T15 | 6ml syringe on a female luer with 15cm sealed SCD tubing | 25个/箱 |
OriGen | 15-RF60-T | 60ml syringe on a female luer with 15cm sealed SCD tubing | 25个/箱 |
OriGen | 15-RF12-T | 12ml syringe on a female luer with 15cm sealed SCD tubing | 25个/箱 |
OriGen | 15-RF20-T | 20ml syringe on a female luer with 15cm sealed SCD tubing | 25个/箱 |
OriGen | R3F-T15 | 3ml syringe on a female luer with 15cm sealed SCD tubing | 25个/箱 |
OriGen | 2S-P20 | Two spikes to one spike port with 20cm SCD tubing and pinch clamps | 20个/箱 |
OriGen | S-38B12 | One spike to one 3/8″ barb with 12cm DMSO resistant tubing, 100% DMSO Resistant | 25个/箱 |
OriGen | S-FFP12 | One spike to one spike port and two female luers with 12cm SCD tubing and pinch clamps | 20个/箱 |
OriGen | 4S-4M60 | Four spikes to four male luers with 60cm SCD tubing, roller and pinch clamps | |
OriGen | VSV | Vial spike with needle-free valve | 10个/包 |
OriGen | S-M50 | One spike to male luer with 50cm SCD tubing and pinch clamp | 50个/箱 |
OriGen | S-F50 | One spike to one female luer with 50cm SCD tubing and pinch clamp | 50个/箱 |
OriGen | S-S50 | One spike to one spike with 50cm of SCD tubing | 12个/箱 |
OriGen | CC2 | Cell Connect One spike and male luer to a 3-way stopcock, 60ml syringe and needle-free valve to four male luers with 70cm SCD tubing and pinch clamps | 12个/箱 |
OriGen | 12-F-M | One female luer to one male luer with 12cm tubing, 100% DMSO resistant | 50个/箱 |
OriGen | CBS | One spike with needle-free valve, 100% DMSO Resistant | |
淀粉 | 货号 | 名称 | 规格 |
B.Braun | L6511 | 6%Hetastarch in 0.9%Sodium Chloride羟乙基淀粉氯化钠 | 500ml/袋 |
B.Braun | L6511 | 6%Hetastarch in 0.9%Sodium Chloride羟乙基淀粉氯化钠 | 12*500ml/箱 |
Hospira | 0409-7419-14-bag | 10% LMD in 0.9%Chlorde Injection右旋糖酐 | 500ml/袋 |
袋子 | 货号 | 名称 | 规格 |
OriGen | EV120+F-M-12 | EVO Bag, 70-120ml Fill Volume, with luer connections and needle free valve with separate female to male tube set with 12cm of tubing | 20套/箱 |
OriGen | EV1000N | EVO Bag,100 -500ml Fill Volume, with one male luer with cap, two female luers with caps, and a needle-free injection port | 12个/箱 |
OriGen | EV3000N | EVO Bag, 500-2000ml Fill Volume, with one male luer with cap, two female luers with caps, and a needle-free injection port | 12个/箱 |