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origen 现货热卖,欢迎咨询!!!

时间:2020-11-10      阅读:1369

 ORIGEN 授权代理,种类齐全,现货供应,现双十一*,*销售,欢迎咨询!!!

 背景知识:
细胞冻存是细胞保存的主要方法之一。利用冻存技术将细胞置于-196℃液氮中低温保存,可以使细胞暂时脱离生长状态而将其细胞特性保存起来,这样在需要的时候再复苏细胞用于实验。而且适度地保存一定量的细胞,可以防止因正在培养的细胞被污染或其他意外事件而使细胞丢种,起到了细胞保种的作用。除此之外,还可以利用细胞冻存的形式来购买、寄赠、交换和运送某些细胞。 细胞冻存时向培养基中加入保护剂--终浓度5%.15%的甘油或二甲基亚砜(DMSO),可使溶液冰点降低,加之在缓慢冻结条件下,细胞内水分透出,减少了冰晶形成,从而避免细胞损伤。 采用"慢冻快融"的方法能较好地保证细胞存活。标准冷冻速度开始为-1 到 -2℃/min,当温度低于-25℃时可加速,到-80℃之后可直接投入液氮内(-196℃)。
二甲基亚砜(DMSO)是一种含硫有机化合物,常温下为无色无味的透明液体,是一种吸湿性的可燃液体,具有高极性、高沸点、热稳定性好、非质子、与水混溶的特性,能溶于乙醇、丙醇、苯等大多数有机物,被誉为“万能溶剂”。二甲基亚砜是一种重要的渗透性细胞保护剂。在深低温(零下200度)保存细胞时冻存过程中,为防止细胞内液冰晶形成、渗透压改变、细胞结构紊乱等导致的损伤,有必要使用含有DMSO冷冻保护剂。DMSO能够快速穿透细胞膜进入细胞中,降低冰点,延缓冻存过程,同时提高细胞内离子浓度,减少细胞内冰晶的形成,从而减少细胞损伤。深低温时二甲基亚砜的细胞毒性受到抑制。复苏时动作要快,尽快洗掉二甲基亚砜,否则会造成对细胞严重的毒性。二甲基亚砜(DMSO)是目前的细胞冻存保护剂,但也是一种细胞毒性很大的化学试剂。研究结果表明,培养液中DMSO浓度为10%时,细胞生长抑制率近100%;1%浓度时抑制率为35%,即使是0.04%的浓度,DMSO对细胞的生长也有不利的影响。
右旋糖酐系蔗糖是经肠膜状明串珠菌-1226(Leuconostoc mesenteroides)发酵合成的一种高分子葡萄糖聚合物,右旋糖酐-40(Dextran-40)别称低分子右旋糖酐,右旋糖酐40的重均分子量(Mw)应为32000~42000。右旋糖酐-40可以维持渗透压和 PiH , 提供适宜细胞存活的介质环境。
OriGen公司研发的二甲亚砜(DMSO)在高级别DMSO的精制领域中处于地位,其细胞冻存级产品在市场上拥有较高的占有率,经过十多年的客户检验与使用,质量过硬。

  ORIGEN 授权代理,种类齐全,现货供应,现双十一*,*销售,欢迎咨询!!!

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冻存袋

货号名称规格
OriGenCS25FFreezing Bags,vol-max,10-25mL冻存袋24个/箱
OriGenCS25NFreezing Bags,vol-max,10-25mL冻存袋24个/箱
OriGenCS50Freezing Bags,vol-max,10-30mL冻存袋24个/箱
OriGenCS50NFreezing Bags,vol-max,10-30mL冻存袋,N型管路 
OriGenCS250Freezing Bags,vol-max,30-70mL冻存袋24个/箱
OriGenCS250SFreezing Bags,vol-max,30-70mL冻存袋12个/箱
OriGenCS250NFreezing Bags,vol-max,30-70mL冻存袋,N型管路24个/箱
OriGenCS500Freezing Bags,vol-max,55-100mL冻存袋24个/箱
OriGenCS750Freezing Bags,vol-max,80-190mL冻存袋24个/箱
OriGenCS1000N Freezing Bags,vol-max,120-275mL冻存袋,N型管路24个/箱
OriGenCS2000N Freezing Bags,vol-max,180-450mL冻存袋24个/箱
OriGenCS2000NP (厂家停产)Freezing Bags,vol-max,180-450mL冻存袋12个/箱
OriGenCS30M95腔室Cryostore Multi-Chamber Bag 12个/箱
OriGenCS24M94腔室Cryostore Multi-Chamber Bag 12个/箱
    
培养袋货号名称规格
OriGenPL07-2GPermaLife Cell Culture Bags1个/包
OriGenPL30-2GPermaLife Cell Culture Bags1个/包
OriGenPL70-2GPermaLife Cell Culture Bags1个/包
OriGenPL120-2GPermaLife Cell Culture Bags1个/包
OriGenPL240-2GPermaLife Cell Culture Bags1个/包
OriGenPL240KPermaLife Cell Culture Bags,定制1个/包
OriGenPL325KPermaLife Cell Culture Bags,定制1个/包
OriGenPL325-2GPermaLife Cell Culture Bags1个/包
OriGenPL500P-2G PermaLife Cell Culture Bags(50个起订)1个/包
OriGenPL750-2GPermaLife Cell Culture Bags1个/包
OriGenPL1000P-2G PermaLife Cell Culture Bags1个/包
OriGenPL2000P2-2G PermaLife Cell Culture Bags(50个起订)1个/包
    
冻存液DMSO货号名称规格
OriGenCP-10Vial, 10ml CryoPur   : 100% DMSO12瓶/盒
OriGenCP-50Vial, 50ml CryoPur   : 100% DMSO6瓶/盒
OriGenCP-70Vial, 70ml CryoPur   : 100% DMSO6瓶/盒
OriGenCP-70-BagVial, 70ml CryoPur   : 100% DMSO70ml/瓶
OriGenCP-100Vial, 100ml CryoPur   : 100% DMSO6瓶/盒
OriGenSP-1010ml in 20ml syringe with stopcock and SCD tubing 1 unit 
OriGenSP-2020ml in 20ml syringe with stopcock and SCD tubing 1 unit 
OriGenSP-3030ml in 20ml syringe with stopcock and SCD tubing 1 unit 
OriGenSP-5050ml in 20ml syringe with stopcock and SCD tubing 1 unit 
    
混合液货号名称规格
OriGenCD-07CryoPur-D™ : 55% DMSO, 5% Dextran-40 w/v, remainder water for injection 
OriGenCD-35CryoPur-D™ : 55% DMSO, 5% Dextran-40w/v, remainder water for injection 
OriGenCD-50CryoPur-D™ : 55% DMSO, 5% Dextran-40 w/v, remainder water for injection6瓶/盒
OriGenCD-50-BagCryoPur-D™ : 55% DMSO, 5% Dextran-40 w/v, remainder water for injection50ml/瓶
OriGenCD-100-BagCryoPur-D™ : 55% DMSO, 5% Dextran-40 w/v, remainder water for injection100ml/瓶
OriGenSD-07CryoPur-D™ : 55% DMSO, 5% Dextran-40w/v, remainder water for injection,带注射器 
OriGenSD-10CryoPur-D™ : 55% DMSO, 5% Dextran-40 w/v, remainder water for injection,带注射器 
OriGenSDS-05A-B5ml 55% DMSO, 5% Dextran 40 w/v, remainder 0.9% saline + 2ml air in  20ml syringe with stopcock and SCD tubing  
OriGenSDS-15A15ml 55% DMSO, 5% Dextran 40 w/v, remainder 0.9% saline in 20ml syringe with stopcock and SCD tubing 
    
外袋货号名称规格
OriGenOW0419 10个/包
OriGenOW0909O-Wrap Overwrap Bags 外袋(适合CS25)10个/包
OriGenOW0918-SO-Wrap Overwrap Bags 外袋(适合CS25,CS50,PL07)10个/包
OriGenOW0918*O-Wrap Overwrap Bags 外袋(适合CS25,CS50,PL07)10个/包
OriGenOW0918-100SO-Wrap Overwrap Bags 外袋(适合CS25,CS50,PL07)100个/箱
OriGenOW1430O-Wrap Overwrap Bags 外袋(适合CS250,CS750,PL30,PL240)10个/包
OriGenOW1936O-Wrap Overwrap Bags 外袋(适合CS1000)10个/包
OriGenOW1950O-Wrap Overwrap Bags 外袋10个/包
OriGenOW2436O-Wrap Overwrap Bags 外袋(适合CS2000N)10个/包
*备注:OW0918*是由100个每箱的大包装分装成10个每包的小包装 
    
热合仪货号名称规格
OriGenCR6 Biosealer CR 6 热合仪 1台/箱
    
OriGenSZ1823SteriZip Overpouch(CS25, CS50 )10个/包
OriGenSZ2533SteriZip Overpouch(CS250, CS500, CS750 )10个/包
OriGenSZ3038SteriZip Overpouch(CS1000, CS2000)10个/包
    
OriGenSZ2533-2M SteriZip Overpouch,2.0毫米 
OriGenSZ2533P-1 SteriZip Overpouch,独立包装50个/包
OriGenSZ2533P-3SteriZip Overpouch,3个/小包45个/包
    
配件货号名称规格
OriGenS-F10One spike to one female luer with 10cm of tubing and pinch clamp, 100% DMSO Resistant50个/箱
OriGenRF-T156ml syringe on a female luer with 15cm sealed SCD tubing25个/箱
OriGen15-RF60-T60ml syringe on a female luer with 15cm sealed SCD tubing25个/箱
OriGen15-RF12-T12ml syringe on a female luer with 15cm sealed SCD tubing25个/箱
OriGen15-RF20-T20ml syringe on a female luer with 15cm sealed SCD tubing25个/箱
OriGenR3F-T153ml syringe on a female luer with 15cm sealed SCD tubing25个/箱
OriGen2S-P20Two spikes to one spike port with 20cm SCD tubing and pinch clamps20个/箱
OriGenS-38B12One spike to one 3/8″ barb with 12cm DMSO resistant tubing, 100% DMSO Resistant25个/箱
OriGenS-FFP12One spike to one spike port and two female luers with 12cm SCD tubing and pinch clamps20个/箱
OriGen4S-4M60Four spikes to four male luers with 60cm SCD tubing, roller and pinch clamps 
OriGenVSVVial spike with needle-free valve10个/包
OriGenS-M50One spike to male luer with 50cm SCD tubing and pinch clamp50个/箱
OriGenS-F50One spike to one female luer with 50cm SCD tubing and pinch clamp50个/箱
OriGenS-S50One spike to one spike with 50cm of SCD tubing12个/箱
OriGenCC2Cell Connect One spike and male luer to a 3-way stopcock, 60ml syringe and needle-free valve to four male luers with 70cm SCD tubing and pinch clamps12个/箱
OriGen12-F-MOne female luer to one male luer with 12cm tubing, 100% DMSO resistant50个/箱
OriGenCBSOne spike with needle-free valve, 100% DMSO Resistant 
    
淀粉货号名称规格
B.BraunL65116%Hetastarch in 0.9%Sodium Chloride羟乙基淀粉氯化钠500ml/袋
B.BraunL65116%Hetastarch in 0.9%Sodium Chloride羟乙基淀粉氯化钠12*500ml/箱
Hospira0409-7419-14-bag10% LMD in 0.9%Chlorde Injection右旋糖酐500ml/袋
    
    
袋子货号名称规格
OriGenEV120+F-M-12EVO Bag, 70-120ml Fill Volume, with luer connections and needle free valve with separate female to male tube set with 12cm of tubing20套/箱
OriGenEV1000NEVO Bag,100 -500ml Fill Volume, with one male luer with cap, two female luers  with caps, and a needle-free injection port12个/箱

OriGen

EV3000NEVO Bag, 500-2000ml Fill Volume, with one male luer with cap, two female luers with caps, and a needle-free injection port12个/箱

 

 

上一篇: RNA完整 BCT™:一种新型的收集管瞄准循环的 RNA 和细胞外囊泡 下一篇: 高起始量带分子标签的DNA-Seq分析
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