anti-Flag Tag Mouse Monoclonal antibody(鼠单克隆抗体)

M1001060anti-Flag Tag Mouse Monoclonal antibody(鼠单克隆抗体)

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2024-02-26 12:59:24
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北京索莱宝科技有限公司

北京索莱宝科技有限公司

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产品简介

anti-Flag Tag Mouse Monoclonal antibody(鼠单克隆抗体)在Western Blotting中使用内参其实就是在WB过程中的另外用内参对应的抗体检测内参,这样在检测目的产物的同时可以检测内参的表达,由于内参在各组织和细胞中的表达相对恒定,借助检测每个样品内参的量就可以用于校正上样误差,这样半定量的结果才更为可信。

详细介绍

英文名称      anti-Flag Tag Mouse Monoclonal antibody

应用             WB 1:2500 IP 1:100 IF:1:500 Optimal dilutions should be determined by the end user.

规  格           100ul

浓  度           1mg/ml

保  存           Store at -20°C. Do not aliquot the antibody.

储存液        PBS, pH 7.4, containing 0.02% sodium azide as Pretive and 50% Glycerol

纯化方法     affinity purified by Protein A

亚  型           IgG

性  状           Liquid

克隆类型      monoclonal

宿  主           Mouse

The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.

说明书

Anti- Flag Tag Mouse Monoclonal Antibody (1B3)

Catalog No. M1001060

Size100μl

Storage: Store at -20°C. Do not aliquot the antibody.

The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to

interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively

as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to

separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can

also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many

different assays that require recognition by an antibody. If there is no antibody against the studied protein,

adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag

sequence.

Molecular Weight: N/A

Application: WB 1:2500 IP 1:100 IF:1:500 Optimal dilutions should be determined by the end user.

Specificity: The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag

fusion proteins.

Form: Liquid ,1mg/ml

Storage buffer: PBS, pH 7.4, containing 0.02% sodium azide as Pretive and 50% Glycerol

Relative Products

M1001010 Anti- HA Tag Mouse Monoclonal Antibody(2C7)

M1001020 Anti-His Tag Mouse Monoclonal Antibody(3C5)

M1001030 Anti-GFP Tag Mouse Monoclonal Antibody(2H7)

M1001040 Anti-GST Tag Mouse Monoclonal Antibody(2C7)

M1001050 Anti- cMyc Tag Mouse Monoclonal Antibody(2C5)

M1001060 Anti- Flag Tag Mouse Monoclonal Antibody(1B3)

RG001010 Anti- HA Tag Rabbit Polyclonal Antibody

RG001020 Anti- His Tag Rabbit Polyclonal Antibody

RG001030 Anti- GFP Tag Rabbit Polyclonal Antibody

RG001040 Anti- GST Tag Rabbit Polyclonal Antibody

RG001050 Anti- cMyc Tag Rabbit Polyclonal Antibody

RG001060 Anti- Flag Tag Rabbit Polyclonal Antibody

Solarbio

Beijing Solarbio Science & Technology Co., Ltd

/82

Http://www.solarbio。。com

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