详细介绍

美国CYTOO细胞微纳米结构图案多孔板和芯片与xonamicrofluidics品牌微流控细胞微图案化培养和分化、相互作用资料

美国CYTOO细胞微纳米结构图案多孔板和芯片应用文献
xonamicrofluidics品牌微流控细胞微图案化产品应用文献

基底材料的拓扑形貌是影响细胞行为的重要因素之一,细胞与微纳米结构图案表面相互作用研究在细胞生物学、再生医学和组织工程领域具有重要的意义。材料表面微纳米图案化不但可以提供结构模版,用以研究细胞对生长环境的响应特性,而且可以为组织再生用支架和植入性器件的设计提供基础数据。

实验结果显示,细胞会根据材料基底表面的图案结构产生相应的形变,而且这种形变响应与图案几何形状、尺寸,细胞伸展和基底表面的黏附蛋白浓度有关。在一定范围内,图案越大、越深,细胞形变越大；细胞伸展面积越大,形变越小：黏附蛋白可以促进细胞伸展,从而使细胞形变减小.微米坑图案能够明显促进细胞在材料表面的黏附；微米沟槽图案 则对细胞具有接触诱导效应,可以诱导细胞响应所生长的微纳米沟槽结构进行骨架重排,从而引起细胞取向行为变化.

CYTOO品牌的细胞微图案产品包括CYTOOPLATE?细胞微图案微孔培养板和CYTOOChips细胞微图案芯片:

CYTOOPLATE?细胞微图案微孔培养板是一个高清晰度的低荧光硼硅玻璃底细胞光刻微图案， CYTOOChips细胞微图案芯片

可以控制细胞粘附性状、大小、细胞培养分化等，研究细胞取，这种方法可以得到稳定的具有显著细胞黏附反差的 "微图案化表面. 利用该图案化表面技术引导细胞黏附后的取向，先在细胞无法贴附的Cytophobic 涂料处理，再以光蚀刻 "的技术，精准地留下特定的几何形狀，并在这样的几形状上涂上特定的细胞间质蛋白(extracellular " matrix , ECM, such as fibronecin, collgene, Laminin, ect…) 。这样的细胞培养工具借由精准的控制细胞贴附的型式，提供了体外培养细胞结构上的引导，使细胞表现出更接近体内生理的表型及框架，这样的工具可以:
◆提高试验的再现性，灵敏性和归纳量化复杂的生物性狀
◆进一步分析细胞结构机械性刺激和细胞功能之间的关系
◆模拟重现细胞在生物体内的环境，并使性狀易于观察分析

"CYTOO 细胞微图案(CYTOO micropattern)使体外培养细胞重现体内应有的细胞极性，有组织的整齐排列，细胞移动的限制!

CYTOO 细胞粘附微图案

量化活细胞分析的突破
精准的对照条件在CYTOO 阵列上，细胞呈现相同的结构

标准的培养皿中的细胞 CYTOO培养产品中的细胞
"细胞贴附在涂有细胞间质蛋白的微图案(micropattern)阵列上时，会随着微图案（micropattern）的引导在不能贴附的材质上张开成特定的几何结构。这样特定形状的贴附接触分布，促使细胞产生固定且再现性*的极性。细胞位置、细胞形状、细胞极性、以及胞器位置都可标准化.
重现体内细胞的物理环境
使体外培养细胞的生理性狀更接近体内组织

CYTOOchipTM细胞微图案芯片
19.5x19.5 mm 盖玻片，由光刻法在高质低荧光硼硅酸盐玻璃上刻画微图形，用于倒置显微镜， "网状结构打印在芯片底部，使用户可以随时找到所需细胞。这些芯片与CYTOOchamber芯片槽结合可实现高分辨率活细胞成像。
标准芯片和平板可用于所有一般用途，初始包的设计是用来开发微图形形状和尺寸的， Arena芯片分为不同尺寸的磁盘，客户定制产品可以使您达到*实验方案。

CYTOOchips细胞微图案芯片客户定制产品包括19.5x19.5 mm 盖玻片，客户可定制微图形，且粘附蛋白可适用于多种应用和实验。定制CYTOOchipszui小量为1组18个芯片，且包裹纤连蛋白或活性表面。
客户定制范围包括：

在我们的标准几何图形的基础上，更大、更小、或密度不同
混合不同的微图形形状
调整芯片表面、面积或形状，方便研究几何图形如何影响细胞结构和行为
平行放置不同微图形
zui低线宽3微米，点5微米

产品规格

CYTOOCHIPS细胞微图案芯片,一包18个
产品名称	货号	(PATTERN)图案	SIZE规格	COATING 涂层
CYTOOCHIPS? MOTILITY A X18	10-031-00-18	Multiple	Multiple	Activated
CYTOOCHIPS? ARENA A X18	10-020-00-18	Disc	Multiple	Activated
CYTOOCHIPS? CUSTOM A X18	10-950-00-18	Multiple	Custom	Activated
CYTOOCHIPS? L-L-A X18	10-015-00-18	L	Large (1600 μm²)	Activated
CYTOOCHIPS? L-S-A X18	10-013-00-18	L	Small (700 μm²)	Activated
CYTOOCHIPS? L-M-A X18	10-014-00-18	L	Medium (1100 μm²)	Activated
CYTOOCHIPS? STARTER'S A X18	10-900-00-18	Multiple	Multiple	Activated
CYTOOCHIPS? DC-S-A X18	10-001-00-18	Disc	Small (700 μm²)	Activated
CYTOOCHIPS? DC-M-A X18	10-002-00-18	Disc	Medium (1100 μm²)	Activated
CYTOOCHIPS? DC-L-A X18	10-003-00-18	Disc	Large (1600 μm²)	Activated
CYTOOCHIPS? CW-L-A X18	10-006-00-18	Crossbow	Large (1600 μm²)	Activated
CYTOOCHIPS? CW-S-A X18	10-004-00-18	Crossbow	Small (700 μm²)	Activated
CYTOOCHIPS? CW-M-A X18	10-005-00-18	Crossbow	Medium (1100 μm²)	Activated
CYTOOCHIPS? Y-L-A X18	10-012-00-18	Y	Large (1600 μm²)	Activated
CYTOOCHIPS? Y-M-A X18	10-011-00-18	Y	Medium (1100 μm²)	Activated
CYTOOCHIPS? Y-S-A X18	10-010-00-18	Y	Small (700 μm²)	Activated
CYTOOCHIPS? H-L-A X18	10-009-00-18	H	Large (1600 μm²)	Activated
CYTOOCHIPS? H-M-A X18	10-008-00-18	H	Medium (1100 μm²)	Activated
CYTOOCHIPS? H-S-A X18	10-007-00-18	H	Small (700 μm²)	Activated

CYTOOplates细胞微图案培养板

CYTOOplates平板为标准SBS微平板版式，由光刻法在高质低荧光硼硅酸盐玻璃底部刻画微图形，可适用于高内涵筛选，且可适用于所有普通HCS成像平台和实验室自动化仪器。
"CYTOOplates客户定制产品包括96孔标准SBS版式，客户可定制微图形，且粘附蛋白可适用于多种应用和实验， "无论所需的微图形形状、尺寸或96孔布局如何。定制zui小量为10个平板，且包裹纤连蛋白或活性表面。
客户定制范围包括:

在我们的标准几何图形的基础上，更大、更小、或密度不同
混合不同的微图形形状
调整平板表面、面积或形状，方便研究几何图形如何影响细胞结构和行为
平行放置不同微图形
zui低线宽3微米，点5微米

CYTOOPLATES细胞微图案培养板订货信息,一包5块
产品名称	货号	(PATTERN)图案	SIZE规格	COATING涂层
CYTOOPLATES? 96 RW L-S-A	20-013-00	L	Small (700 μm²)	Activated
CYTOOPLATES? 96 RW L-L-A	20-015-00	L	Large (1600 μm²)	Activated
CYTOOPLATES? 96 RW L-M-A	20-015-00	L	Medium (1100 μm²)	Activated
CYTOOPLATES? 96 RW DC-S-A	20-001-00	Disc	Small (700 μm²)	Activated
CYTOOPLATES? 96 RW DC-M-A	20-002-00	Disc	Medium (1100 μm²)	Activated
CYTOOPLATES? 96 RW DC-L-A	20-003-00	Disc	Large (1600 μm²)	Activated
CYTOOPLATES? 96 RW CW-S-A	20-004-00	Crossbow	Small (700 μm²)	Activated
CYTOOPLATES? 96 RW CW-M-A	20-005-00	Crossbow	Medium (1100 μm²)	Activated
CYTOOPLATES? 96 RW CW-L-A	20-006-00	Crossbow	Large (1600 μm²)	Activated
CYTOOPLATES? 96 RW H-S-A	20-007-00	H	Small (700 μm²)	Activated
CYTOOPLATES? 96 RW H-M-A	20-008-00	H	Medium (1100 μm²)	Activated
CYTOOPLATES? 96 RW H-L-A	20-009-00	H	Large (1600 μm²)	Activated
CYTOOPLATES? 96 RW Y-S-A	20-010-00	Y	Small (700 μm²)	Activated
CYTOOPLATES? 96 RW Y-M-A	20-011-00	Y	Medium (1100 μm²)	Activated
CYTOOPLATES? 96 RW Y-L-A	20-012-00	Y	Large (1600 μm²)	Activated
CYTOOPLATES? 96 RW STARTER	20-900-00	Multiple	Multiple	Activated
CYTOOPLATES? 96 RW MOTILITY A	20-031-00	Line	Multiple	Activated

CYTOO chamber芯片槽
单孔芯片槽提供了大面积的成像区域，可承装10,000个细胞图形，这款产品尤其适合当您有超过4 "个不同的几何图形打印在芯片上的时候，以及像是有丝分裂的细胞的特例测量。次产品更适用于高分辨率活细胞成像。
4孔芯片槽可提供每孔上千种图形在各情境下的平行免疫荧光实验，Arena芯片和Motility芯片的4象限设计使之适用于4孔芯片槽。
优势和主要特征:

可重复使用，高温灭菌
磁性装置使用简单
35mm足迹
与普通显微镜台兼容
zui多四平行

CYTOO chamber灌流槽
"闭合单通道或双通道CYTOOchamber灌流槽用于活细胞成像,使用简单,专门设计用于我们的CYTOOchips芯片. 主要部分为透明的丙烯酸材料，底板是黑色的阳极化处理铝合金，部件还包括一圈硅垫。通道尺寸可根据要求定制。
优势和主要特征：

- 闭合灌流槽
- 培养基补充简易
- 与CYTOOchips兼容
- 磁性装置使用简单
- 可重复利用
- 单双孔道标准配置，客户定制通道尺寸
- 可配置显微镜台适配器

Xonamicrofluidics品牌细胞微沟槽图案流体培养板
货号	产品名称	图片
RD150	Round Device (150 um microgroove barrier) Size: ~ 21 mm diameter Our round device version of the standard 150 um microgroove barrier has an approximate diameter of 21 mm, making it compatible with some 35 mm glass bottom dishes that many researchers use in live cell imaging systems. Glass bottom dishes used should have an inner aperture of 21 mm or more to accommodate the device. At least 22 mm is recommended in order to have room to position the device. Larger glass bottom dishes can be used, as well as glass cover slips, if desired.
RD450	Round Device (450 um microgroove barrier) Size: ~ 21 mm diameter Our round device version of the standard 450 um microgroove barrier has an approximate diameter of 21 mm, making it compatible with some 35 mm glass bottom dishes that many researchers use in live cell imaging systems. Glass bottom dishes used should have an inner aperture of 21 mm or more to accommodate the device. At least 22 mm is recommended in order to have room to position the device. Larger glass bottom "dishes can be used, as well as glass cover slips, if desired.
RD900	Round Device (900 um microgroove barrier) Size: ~ 21 mm diameter Our round device version of the standard 900 um microgroove barrier has an approximate diameter of 21 mm, making it compatible with some 35 mm glass bottom dishes that many researchers use in live cell imaging systems. Glass bottom dishes used should have an inner aperture of 21 mm or "more to accommodate the device. At least 22 mm is recommended in order to "have room to position the device. Larger glass bottom dishes can be used, as "well as glass cover slips, if desired.
SND150	Standard Neuron Device (150 um microgroove barrier) Size: ~ 22 mm – ~23mm The 150 um neuron device with its shorter microgroove barrier is suitable for researchers who would like to isolate both axons and dendrites. It is also suitable for certain "neuronal cultures that do not grow long processes. Researchers who wish to study the early events of axonal and dendritic growth "may also find the 150 um device useful. The 150 um device "also offers the benefits of fluidic isolation and culture organization for transport studies.
SND450	Standard Neuron Device (450 um microgroove barrier) Size: ~ 22 mm – ~23mm "The 450 um neuron device is our most popular device. For most "applications it is suitable for separating cell bodies from axons. After two weeks, dendrites in E18 rat cortical neurons have typically not crossed the microgroove barrier. "The 450 um neuron device also provides the added features of fluidic "isolation and culture organization for transport studies.
SND900	Standard Neuron Device (900 um microgroove barrier) Size: ~ 22 mm – ~23mm The 900 um neuron device, with its wide microgroove barrier, is suitable for researchers who would like to perform long term experiments while ensuring axonal isolation. "In some cultures, after two weeks of growth dendrites may cross the 450 um barrier thereby necessitating the use of the 900 um device.It is also suitable "for certain neuronal cultures which grow long processes. Researchers who "also wish to make sure they are isolating * axons only in the distal "chamber may also find the 900 um device useful. The 900 um device also offers the benefits of fluidic isolation and culture organization for transport studies.
TCND500	Triple Chamber Neuron Device (500 um microgroove barrier) Size: ~ 38 mm – ~24mm The TCND500 consists of (2) 500 um microgroove barriers with a 500 um central chamber. This device makes it possible to culture cortical "neurons in the left and right chambers while culturing hippocampal neurons, or other combinations of cells, in the middle. The triple chamber is also suitable for transport studies where the researcher wishes to treat the middle of the axon growing in the middle chamber while observing effects at the growth cone or in the somal compartment. Neurons can also be loaded in the central chamber and different treatments applied to the left and right compartments.
TCND1000	Triple Chamber Neuron Device (1000 um microgroove barrier) Size: Size: ~ 38 mm – ~24mm The TCND1000 consists of (2) 500 um microgroove barriers with a 1000 um central chamber. This device makes "it possible to culture cortical neurons in the left and "right chambers while culturing hippocampal neurons, or other combinations of cells, in the middle. The triple chamber is also suitable for transport studies where the researcher wishes to treat the middle of the axon growing in the middle chamber while observing effects at the growth cone or in the somal compartment. Neurons can also be loaded in the central chamber and different treatments "applied to the left and right compartments.
uLP	Microfluidic Local Perfusion Device barrier, (50 um microgroove 75 micron perfusion channel, 500 um microgroove barrier) Size: ~ 40 mm – ~24 mm "Our Microfluidic Local Perfusion Chamber was first published in Neuron. "2010 Apr 15;66(1):57-68. This device has a 75 micron perfusion "chamber with a set of microgrooves on the left 50 um in width and a set of "microgrooves on the right 500 um in width. This device, in conjuction with a "syringe pump or other suitable equipment, allows the researcher to perfuse "selected treatments and compounds across the target area. Syringe pump or "other suitable equipment required for proper use. For more indepth "information please see Neuron. 2010 Apr 15;66(1):57-68.