Biocolor 弹性蛋白检测试剂盒
Biocolor Fastin elastin Assay 

利用染料结合的方法来定量检测释放到组织培养液或从生物材料中提取的弹性蛋白。
 
应用范围：样本可来源于细胞培养基和含细胞外基质成分的组织提取物（如弹性软骨、主动脉、皮肤等），可检测可溶性弹性蛋白原和不可溶性弹性蛋白（抽提后以可溶性弹性蛋白多肽的形式检测）。
 
样本要求：样本体积5-100ul，包含5-75ug的弹性蛋白
 
实验操作步骤

弹性蛋白的分离：用弹性蛋白沉淀剂处理样本，使弹性蛋白沉淀下来。
弹性蛋白与染料结合：装有沉淀的弹性蛋白管中，加染料和反应液，反应60min以上
弹性蛋白-染料复合物获得：去掉未结合的染料
复合物染料的释放：添加染料释放剂，使弹性蛋白-染料复合物中的染料释放出来
释放出的染料检测：绘制标准曲线，513nm或405nm处定量检测

产品订购

   Product Code: F2000        110 assays

   Product Code: F4000        440 assays

参考文献

1
Allen, R. A., Wu, W., Yao, M., Dutta, D., Duan, X., Bachman, T. N., Champion, H. C., Stolz, D. B., Robertson, A. M.,
Kim, K., et al. (2014) Nerve regeneration and elastin formation within poly (glycerol sebacate)-based synthetic arterial grafts one-year post-implantation in a rat model. Biomaterials 35, 165–173.
2
Anilkumar, T. V., Vineetha, V. P., Revi, D., Muhamed, J. and Rajan, A. (2014) Biomaterial properties of cholecystderived scaffold recovered by a non-detergent/enzymatic method. Journal of Biomedical Materials Research Part B:
Applied Biomaterials.
3
AUGUSTIN, C., FREI, V., PERRIER, E., HUC, A. and DAMOUR, O. AN IN VITRO SELECTION OF NEW COSMETIC ACTIVE
COMPOUNDS: FROM SCREENING TESTS ON MONOLAYERED FIBROBLAST CULTURE TO EFFICIENCY STUDY ON 3-D DERMAL
EQUIVALENT. AMMONIO LATTATO" ATTIVATO 1.
4
Baiguera, S., Del Gaudio, C., Kuevda, E., Gonfiotti, A., Bianco, A. and Macchiarini, P. (2014) Dynamic decellularization and cross-linking of rat tracheal matrix. Biomaterials 35, 6344–6350.
5
Baiguera, S., Del Gaudio, C., Lucali, E., Kuevda, E., Boieri, M., Mazzanti, B., Bianco, A. and Macchiarini, P. (2014) Electrospun gelatin scaffolds incorporating rat decellularized brain extracellular matrix for neural tissue engineering. Biomaterials 35, 1205–1214.
6
Cheheltani, R., McGoverin, C. M., Rao, J., Vorp, D. A., Kiani, M. F. and Pleshko, N. (2014) Fourier transform infrared spectroscopy to quantify collagen and elastin in an in vitro model of extracellular matrix degradation in aorta. Analyst 139, 3039–3047.
7
Chen, M.-H., Hu, C.-K., Chen, P.-R., Chen, Y.-S., Sun, J.-S. and Chen, M.-H. (2014) Dose-dependent regulation of cell proliferation and collagen degradation by estradiol on ligamentum flavum. BMC musculoskeletal disorders 15, 238.
8
Chow, M.-J., Turcotte, R., Lin, C. P. and Zhang, Y. (2014) Arterial Extracellular Matrix: A Mechanobiological Study of the Contributions and Interactions of Elastin and Collagen. Biophysical journal 106, 2684–2692.
9 Gilpin, S. E., Guyette, J. P., Gonzalez, G., Ren, X., Asara, J. M., Mathisen, D. J., Vacanti, J. P. and Ott, H. C. (2014) Perfusion decellularization of human and porcine lungs: Bringing the matrix to clinical scale. The Journal of Heart and Lung Transplantation 33, 298–308.
10
Gu, Q., Wang, B., Zhang, X.-F., Ma, Y.-P., Liu, J.-D. and Wang, X.-Z. (2014) Chronic aerobic exercise training
attenuates aortic stiffening and endothelial dysfunction through preserving aortic mitochondrial function in aged rats. Experimental gerontology 56, 37–44.
11
Gu, Q., Wang, B., Zhang, X.-F., Ma, Y.-P., Liu, J.-D. and Wang, X.-Z. (2014) Contribution of renin-angiotensin system to exercise-induced attenuation of aortic remodeling and improvement of endothelial function in spontaneously hypertensive rats. Cardiovascular Pathology.
12
Isayama, N., Matsumura, G., Sato, H., Matsuda, S. and Yamazaki, K. (2014) Histological maturation of vascular smooth muscle cells in in situ tissue-engineered vasculature. Biomaterials 35, 3589–3595.13
Jelinic, M., Leo, C.-H., Uiterweer, E. D. P., Sandow, S. L., Gooi, J. H., Wlodek, M. E., Conrad, K. P., Parkington, H., Tare, M. and Parry, L. J. (2014) Localization of relaxin receptors in arteries and veins, and region-specific increases in compliance and bradykinin-mediated relaxation after in vivo serelaxin treatment. The FASEB Journal 28, 275–287.
14
Larroque-Cardoso, P., Mucher, E., Grazide, M.-H., Josse, G., Schmitt, A.-M., Nadal-Wolbold, F., Zarkovic, K., Salvayre, R. and Nègre-Salvayre, A. (2014) 4-Hydroxynonenal impairs transforming growth factor-β1-induced elastin synthesis via epidermal growth factor receptor activation in human and murine fibroblasts. Free Radical Biology and Medicine 71,
427–436.
15
Latimer, C. A., Nelson, M., Moore, C. M. and Martin, K. E. (2014) Effect of collagen and elastin content on the burst pressure of human blood vessel seals formed with a bipolar tissue sealing system. Journal of Surgical Research 186, 73–80.
16
Liu, S., Parameswaran, H., Young, S. M. and Varisco, B. M. (2014) JNK suppresses pulmonary fibroblast elastogenesis during alveolar development. Respiratory research 15, 34.
17
Merrilees, M. J., Falk, B. A., Zuo, N., Dickinson, M. E., May, B. C. and Wight, T. N. (2014) Use of versican variant V3 and versican antisense expression to engineer cultured human skin containing increased content of insoluble elastin.Journal of tissue engineering and regenerative medicine.
18
Mizuno, M., Takebe, T., Kobayashi, S., Kimura, S., Masutani, M., Lee, S., Jo, Y. H., Lee, J. I. and Taniguchi, H. (2014) Elastic Cartilage Reconstruction by Transplantation of Cultured Hyaline Cartilage–Derived Chondrocytes. In Transplantation proceedings, pp 1217–1221, Elsevier.
19
Price, A. P., Godin, L. M., Domek, A., Cotter, T., D’Cunha, J., Taylor, D. A. and Panoskaltsis-Mortari, A. (2014)
AUTOMATED DECELLULARIZATION OF INTACT, HUMAN-SIZED LUNGS FOR TISSUE ENGINEERING. Tissue Engineering.
20
Santoso, E. G., Yoshida, K., Hirota, Y., Aizawa, M., Yoshino, O., Kishida, A., Osuga, Y., Saito, S., Ushida, T. and Furukawa, K. S. (2014) Application of Detergents or High Hydrostatic Pressure as Decellularization Processes in Uterine Tissues and Their Subsequent Effects on In Vivo Uterine Regeneration in Murine Models. PloS one 9, e103201.
21
Sivan, U., Jayakumar, K. and Krishnan, L. K. (2014) Matrix-directed differentiation of human adipose-derived mesenchymal stem cells to dermal-like fibroblasts that produce extracellular matrix. Journal of tissue engineering and regenerative medicine.
22
Smith, K. D., Clegg, P. D., Innes, J. F. and Comerford, E. J. (2014) Elastin content is high in the canine cruciate ligament and is associated with degeneration. The Veterinary Journal 199, 169–174.
23
Tam, K., Cheyyatraviendran, S., Venugopal, J., Biswas, A., Choolani, M., Ramakrishna, S., Bongso, A. and Fong, C.-Y. (2014) A nanoscaffold impregnated with human Wharton’s Jelly stem cells or its secretions improves healing of wounds. Journal of cellular biochemistry 115, 794–803.