Dynabeads™ Human T-Activator CD3/CD28 免疫磁珠通过偶联CD3/CD28单抗，活化扩增人T细胞。

Dynabeads™ Human T-Activator CD3/CD28 免疫磁珠

产品英文名 Dynabeads™ Human T-Activator CD3/CD28 for T Cell Expansion and Activation

产品中文名 Dynabeads™ Human T-Activator CD3/CD28 免疫磁珠

产品介绍  Dynabeads® Human T-Activator CD3/CD28 用于活化和扩增人 T 细胞和 T 细胞克隆，以作研究之用。Dynabeads® CD3/CD28 T Cell Expander 提供了活化和扩增 T 细胞的简单方法，无需抗原呈递细胞或抗原。 Dynabeads® Human T-Activator CD3/CD28 是均匀的 4.5 µm 超顺磁珠，与抗 CD3 和抗 CD28 单克隆抗体的优化混合物偶联。 通过使抗 CD3 和抗 CD28 抗体与 Dynabeads® 结合，这种微珠可以提供 T 细胞活化和扩增所需的原始和共刺激信号。

Dynabeads免疫磁珠

欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司  ：1500 1904 520。红荣微再-客服:  经销商专员

起始样品

Dynabeads免疫磁珠适用来自全血或血沉棕黄层的 MNC/PBMC，或者包含 CD3+ T 细胞、CD4+ 或 CD8+ T 细胞、调节性 T 细胞或 T 细胞克隆的免疫系统T 细胞亚群。

扩增效果

人T细胞扩增：12天扩增3000倍

规格

Cell Type: T Cells (Whole Population)

Clonality: Monoclonal

Final Product: Cells

Product Line: DYNAL®, Dynabeads®

Reactivity: Human

Sample Type (Specific): Cell Cultures, PBMC

Target Species: Human

Shipping Condition: Room Temperature

细胞类型：免疫T细胞

克隆：单克隆

产物：细胞

产品线：DYNAL®, Dynabeads®

反应性：人

样本类型：细胞培养物，PBMC（外周血单个核细胞）

靶种属：人

运输条件：室温

内容及储存

Dynabeads® Human T-Activator CD3/CD28 are supplied as a suspension containing 4 × 10⁷ Dynabeads®/ml in phosphate buffered saline (PBS), pH 7.4, with 0.1% human serum albumin (HSA). Store at: 2-8°C.

Dynabeads免疫磁珠是一种悬液，4 × 10⁷ Dynabeads®/ml的磁珠溶于PBS，pH 7.4，另含0.1%人白蛋白。

2-8°C储存。

Dynabeads免疫磁珠特点

Dynabeads® Human T-Activator CD3/CD28 are for the activation and expansion of human T cells. Advantages of Dynabeads® Human T-Activator CD3/CD28:

• Activation of T cells without the need for feeder cells

T细胞活化无需滋养层细胞

• Activated cells that retain in vivo-like function

活化细胞功能同体内培养

• Maximum reproducibility without contamination by soluble antibodies or mitogens

zui高可重复性，没有抗体溶解和丝裂原污染。

• From 100- to 1,000-fold T cell expansion

T细胞扩增100-1000倍

特别应用

CAR-T细胞活化扩增：

CAR-T免疫疗法

继诺华和Kite公司两款CAR-T细胞治疗被FDA批准，2017年被媒体评为CAR-T疗法的元年。

CAR T细胞免疫疗法中，因为纯化自体抗原呈递细胞（APCs）用于T细胞活化需要好几个额外步骤，增加获得CAR T细胞产品强度和难度。因此研究者开发了另外一种更标准化、更具效率的T细胞活化方法，比如使用抗CD3/抗CD28单克隆抗体（anti-CD3/anti-CD28 monoclonal antibodies）。

而Dynabeads® Human T-Activator CD3/CD28 这种微珠可以提供 T 细胞活化和扩增所需的原始和共刺激信号，通过使抗 CD3和抗 CD28 抗体与 Dynabeads® 结合，更加速扩增。

推荐PeproTech抗CD3/抗CD28单克隆抗体产品

T细胞活化抗人CD3单克隆抗体（Functional，clone: OKT3）可以与TCR复合物结合，激活T细胞。SAFIRE(Sodium Azide Free Immensely Reduced Endotoxins) 类产品是BioGems品牌下的功能性抗体产品。

欢迎您致电 华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司  ：1500 1904 520。红荣微再-客服:  经销商专员

About Dynabeads® Human T-Activator CD3/CD28

Dynabeads® Human T-Activator CD3/CD28 offer a simple method for activation and expansion of T cells that does not require feeder cells (antigen-presenting cells) or antigen. The uniform 4.5 µm diameter, inert, superparamagnetic beads are similar in size to antigen-presenting cells, and are covalently coupled to anti-CD3 and anti-CD28 antibodies. These two antibodies provide primary and co-stimulatory signals, optimized for efficient T cell activation and expansion. Expansion of the T cell population can be stimulated using recombinant IL-2, and after activation or expansion, the magnetic beads can be easily removed using a DynaMag™ magnet. For expansion of antigen-specific T cells from T cell clones or T cell lines, we recommend using Dynabeads® Human T-Activator CD3/CD28/CD137.

Starting samples

Start with mononuclear cells (MNCs)/peripheral blood mononuclear cells (PBMCs) from either whole blood or buffy coat, or T cell subsets including CD3+ T cells, CD4+ or CD8+ T cells, or regulatory T cells.

Downstream applications

The activated T cells can be analyzed shortly after activation (for transfection/transduction or to study TCR signaling, proteomics, gene expression, etc.). T cells can be left in culture to differentiate into T helper cell subsets or expansion of polyclonal/antigen-specific T cells.

For Research Use Only. Not for use in diagnostic procedures.

引用及参考文献

Activated human CD4+ T cells express transporters for both cysteine and cystine.

Levring TB, Hansen AK, Nielsen BL, Kongsbak M, von Essen MR, Woetmann A, Odum N, Bonefeld CM, Geisler C, Sci Rep (2012) 2:266-266

Dasatinib, a small molecule protein tyrosine kinase inhibitor, inhibits T cell activation and proliferation.

Schade AE, Schieven GL, Townsend R, Jankowska AM, Susulic V, Zhang R, Szpurka H, Maciejewski JP, Blood 2007 0(0):-

Loss of Siglec expression on T lymphocytes during human evolution.

Nguyen DH, Hurtado-Ziola N, Gagneux P, Varki A

Proc Natl Acad Sci U S A (2006) 103:7765-7770