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英文名称：Cellartis RHB-Basa Stem Cell Culture Media，Cellartis RHB-A Stem Cell Culture Media

中文名称：Cellartis RHB-Basa神经干细胞培养基，Cellartis RHB-A神经干细胞培养基  

RHB-A和RHB-Basal是两款无血清、成分*确定的培养基，用于人类或小鼠神经干细胞（NS）的定向分化获取、维持培养、扩增、诱导分化成神经细胞等用途。RHB-A可用来培养扩增贴壁的人或小鼠的神经干细胞。在RHB-A中培养的神经干细胞保持着稳定的神经干细胞分化发育成为成熟的神经细胞的能力 (1-3) 。另外，RHB-A还用来定向分化小鼠胚胎干细胞(mouse ES)至神经前体细胞 (neural precursors) (4-6)。

RHB‐Basal不包含生长因子或是神经细胞相关的添加物。因此，可以RHB‐Basal为基础，通过添加客户认为需要的添加物来开发适合客户的特殊类型细胞的培养基。

RHB-A and RHB-Basal is a fully defined, serum-free cell culture medium for the derivation, maintenance, expansion, and differentiation of human and mouse neural stem (NS) cells. RHB-A is specifically formulated for culturing pure populations of adherent human and mouse NS cells. This optimized medium ensures the long-term stability of NS cell differentiation capacity (1) and can be used to support differentiation of human and mouse NS cells into functional neurons (1–3) and of mouse ES cells into neural precursors (4–6).

RHB‐Basal does not contain any growth factors or neuronal supplements.  Therefore, this media can be tailored to the specific requirements of your cell type by the addition of supplements.

产品特点：(NS细胞：神经干细胞）

RHB-A是一种专有的成分确定的无血清培养基，用于维持人类和小鼠贴壁NS细胞群培养

RHB‐Basal不含动物成分，不含神经元添加剂；培养基可以通过补充添加剂来定制

RHB-A is a proprietary, fully defined, and serum-free medium designed to maintain pure populations of adherent human and mouse NS cells

RHB-Basal medium is animal component-free and contains no neuronal supplements; the media can be customized by the addition of supplements

产品应用(ES：胚胎干细胞）

从ES细胞和胎儿和成人组织中分离培养小鼠和人类NS细胞

小鼠和人类贴壁NS细胞的维持和增殖

小鼠和人类NS细胞分化为功能性神经元

小鼠ES细胞向神经元前体的分化

有关使用的其他示例，请参阅数据表

Derivation of mouse and human NS cells from ES cells and fetal and adult tissues

Maintenance and propagation of adherent mouse and human NS cells

Differentiation of mouse and human NS cells into functional neurons

Differentiation of mouse ES cells to neuronal precursors

Refer to the Data Sheet for additional examples of use

组份：

500 ml medium

参考文献：

1. Sun Y, Pollard S, Conti L, Toselli M, Biella G, Parkin G, Willatt L, Falk A, Cattaneo E, and Smith A. (2008) Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture. Molecular and Cellular Neuroscience. 38: 245-258.

2.Conti L, Pollard SM, Gorba T, Reitano E, Toselli M, Biella G, Sun Y, Sanzone S, Ying QL, Cattaneo E, and Smith A. (2005) Niche-Independent symmetrical self-renewal of a mammalian tissue stem cell. PLoS Biology. 3(9): e283.

3.Pollard SM, Conti L, Sun Y, Goffredo D, and Smith A. (2006) Adherent Neural Stem (NS) cells from fetal and adult forebrain. Cerebral Cortex. 16: 112-120.

4.Ying QL, Stavridis M, Griffiths D, Li M, and Smith A. (2003) Conversion of embryonic stem cells into neuroectodermal precursors in adherent mono-culture. Nature Biotechnology. 21: 183-186.

5.Pollard SM, Wallbank R, Tomlinson S, Grotewold L, and Smith A. (2008) Fibro-blast growth factor induces a neural stem cell phenotype in foetal forebrain progenitors and during embryonic stem cell differentiation. Molecular and Cellular Neuroscience. 38: 393-403.

6.Pollard SM, Yoshikawi K, Clarke ID, Danovi D, Stricker S, Russell R, Bayani J, Head R, Lee M, Bernstein M, Squire J, Smith A, and Dirks P. (2009) Glioma stem cell lines expanded in adherent culture have tumor-specific phenotypes and are suitable for chemical and genetic screens. Cell Stem Cell. 4: 568-580.

7.Diogo MM, Henrique D, and Cabral JM. (2008) Optimization and integration of expansion and neural commitment of mouse embryonic stem cells. Bio-technology and Applied Biochemistry. 49: 105-112.

8.Abranches E, Silva M, Pradier L, Schulz H, Hummel O, Henrique D, and Bek-man E. (2009) Neural differentiation of embryonic stem cells in vitro: A road map to neurogenesis in the embryo. PLoS ONE. 4(7): e6286.

9.Hansen DV, Lui JH, Parker PRL, and Kriegstein AR. (2010) Neurogenic radial glia in the outer subventricular zone of human neocortex. Nature. 464(7288): 554-561.

华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司主营细胞治疗和精准医疗等产品，2018年与TAKARA继续合作，授权代理（上海）其旗下Cellartis干细胞研究制品和Rubicon单细胞文库构建试剂。Cellartis公司位于瑞典，拥有iPS细胞等干细胞分化为肝细胞及脏器细胞的分化诱导技术和ES细胞，ips细胞，分化细胞等干细胞相关产品。

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