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iPS向肝脏细胞定向分化试剂盒

产品英文名称：Cellartis® iPS Cell to Hepatocyte Differentiation System

产品中文名称：iPS向肝脏细胞定向分化试剂盒

iPS向肝脏细胞定向分化各个步骤的培养

产品介绍

以肝脏细胞为模型的实验越来越受到重视，在肝脏病变机理研究、药物代谢研究、药物毒理评估等方面有广阔的应用前景。然而，以个体原代肝细胞为材料来源会产生如取材困难、材料批间差大、无法长期稳定供应等一系列难以克服的问题。因此，通过iPS批量转化生产肝细胞的方法就显示出特别的优势。

Cellartis iPS Cell to Hepatocyte Differentiation System可以稳定地、规模化地实现iPS向hepatocyte的诱导转化生产，而且诱导转化出的肝细胞能够稳定表达药物代谢相关酶系统和药物转运系统。

Cellartis iPS Cell to Hepatocyte Differentiation System是一套Do-It-Yourself系统，客户既可以对某个特殊病人来源的iPS实现转化，也可以利用cellartis供应的iPS进行标准化操作。使用本产品的过程起始于诱导人iPS转化成为确定性内胚层（Definitive Endoderm (DE)）细胞，然后诱导转化DE至肝细胞。本产品是All-In-One型产品，实验所需的培养基、基质（coating）等包括在内。应用本产品转化约3 × 10⁶ hiPS cells,可以获得约5 × 10⁶ 肝细胞（大约50 cm²面积的单层贴壁细胞）。

利用Cellartis iPS Cell to Hepatocyte Differentiation System转化得到的肝细胞除了清晰表现出肝细胞的*特征外（如肝细胞特异性标志物、CYP系列酶等），还保留了原始细胞供体者的遗传基因背景。另外，相比较从组织切除获得的原代肝脏细胞（通常需要低温保存），通过本产品系统转化获得的肝细胞在更长的时间跨度上保持着生理功能的稳定性。这一点对于毒理学评估和病毒感染评估具有显著的意义。

产品特点:

高度可重复的强大系统 - *相同的方法已被证明可用于25种不同的iPS细胞系，因此无需针对您的细胞进行优化。

理想的药物代谢和安全性研究 - 始终如一地生成具有不同遗传背景的> 90％纯的功能性hiPS细胞来源的肝细胞。

定制的起始材料 - 从任何疾病相关的iPS细胞系开始，创建准确的肝脏疾病模型。

准备好个性化医疗 - 为治疗应用制造针对患者的疾病特异性细胞。

扩展实验窗口 - 肝细胞可用于功能测试至少11天。

Highly reproducible, robust system—the exact same protocol has been shown to work across 25 different iPS cell lines, so no need to optimize for your lines.

Ideal for drug metabolism and safety studies—consistently generate panels of >90% pure, functional, hiPS cell-derived hepatocytes with diverse genetic backgrounds.

Customized starting materials—start with any disease-relevant iPS cell lines and create accurate liver disease models.

Ready for personalized medicine—make patient-specific, disease-specific cells for therapeutic applications.

Extended experimental window—hepatocytes can be used for a minimum of 11 days for functional tests.

产品应用:

iPS向肝脏细胞定向分化

组份：iPS细胞培养基+iPS分化内胚层培养基+内胚层分化肝细胞培养基

每种培养基都是*培养基

Cellartis iPS Cell to Hepatocyte Differentiation System (Cat. No. Y30055)：

Cellartis DEF-CS 100 Culture System (Cat. No. Y30020)

▪ 100 ml DEF-CS 100 Basal Medium

▪ 800 µl DEF-CS COAT-1 (for 100 ml)

▪ 300 µl DEF-CS GF-1 (for 100 ml)

▪ 100 µl DEF-CS GF-2 (for 100 ml)

▪ 40 µl DEF-CS GF-3 (for 100 ml)

Cellartis Definitive Endoderm Differentiation Kit (Cat. No. Y30030)

▪ 1 tube Definitive Endoderm Differentiation Coating (10 ml)

▪ 1 bottle Definitive Endoderm Differentiation Day 0 (18 ml)

▪ 1 bottle Definitive Endoderm Differentiation Day 1 (18 ml)

▪ 1 bottle Definitive Endoderm Differentiation Day 2 (18 ml)

▪ 1 bottle Definitive Endoderm Differentiation Day 3 (25 ml)

▪ 1 bottle Definitive Endoderm Differentiation Day 4 (47 ml)

▪ 1 bottle Definitive Endoderm Differentiation Day 6 (47 ml)

Cellartis Hepatocyte Differentiation Kit (Cat. No. Y30050)

▪ 1 bottle Hepatocyte Thawing and Seeding Medium (1) (25 ml)

▪ 1 bottle Hepatocyte Progenitor Medium (2) (50 ml)

▪ 2 bottles Hepatocyte Maturation Medium Base (3A) (24 ml)

▪ 1 tube Hepatocyte Maturation Medium Supplement (3B) (2.6 ml)

▪ 3 bottles Hepatocyte Maintenance Medium (4) (50 ml)

▪ 1 tube Hepatocyte Coating (7.5 ml)

Cellartis-Y30055 iPS Cell to Hepatocyte Differentiation System详询

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自备试剂：

人多能干细胞

胎牛血清（FBS）或血清替代品（KO-SR）

PBS Dulbecco's（含有Ca2 +和Mg2 +的D-PBS + / +）

PBS Dulbecco's不含Ca 2+和Mg 2+（D-PBS - / - ）

TrypLE选择酶（1X），不含酚红

保存：

iPS细胞培养基添加剂–20°C，基础培养基和包被剂2–8°C

iPS分化内胚层培养基和内胚层分化肝细胞培养基所有组份都保存在–20°C。

Cellartis DEF-CS 100 Culture System (Y30020)：DEF-CS 100 Basal Medium ，COAT-1   2–8°C；GF-1, GF-2, and GF-3  –20°C

Cellartis Definitive Endoderm Differentiation Kit (Y30030)：–20°C

Cellartis Hepatocyte Differentiation Kit (Y30050) ：–20°C

产品性能：

Immunocytochemistry analysis of hepatocyte differentiation. hiPS cells were differentiated into functional hepatocytes using the Cellartis iPS Cell to Hepatocyte Differentiation System. Hepatocytes were immunostained to detect early hepatic markers HNF4α (red, nuclear) and CK18 (green) on days 14 and 21. As the hepatocytes matured, expression of liver-specific markers CYP3A (red, cytoplasmic) and Albumin (green) increased as seen on day 28, as expected. Cell nuclei were stained with DAPI (blue).

参考文献：

Asplund, A., et al. (2015) Stem Cell Rev. and Rep. Advance online publication. doi: 10.1007/s12015-015-9621-9

订购信息

肝细胞培养完整解决方案

人iPS诱导分化成肝细胞全过程的完整试剂盒：Y30055（新产品）

人iPS来源肝细胞株及*培养基Y10133/Y10134/Y10135（新产品）

人原代肝脏细胞培养基 Y20020（新产品）

https://www.chem17.com/st321032/article_1496885.html

华雅再生医学旗舰公司：红荣微再（上海）生物工程技术有限公司主营细胞治疗和精准医疗等产品，2018年与TAKARA继续合作，授权代理（上海）其旗下Cellartis干细胞研究制品和Rubicon单细胞文库构建试剂。Cellartis公司位于瑞典，拥有iPS细胞等干细胞分化为肝细胞及脏器细胞的分化诱导技术和ES细胞，ips细胞，分化细胞等干细胞相关产品。

Cellartis-Y30055 iPS Cell to Hepatocyte Differentiation System 手机1500 1904 520 红荣微再-客服  经销商专员