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面议CUTANA pAG-Tn5是靶向剪切及转座酶(CUT&Tag)技术中进行高效绘制染色质特征的关键试剂。与ChIP-seq相比，CUT&Tag在降低细胞需求量和测序深度的信噪比方面进行了显著改进。CUTANA pAG-Tn5是一种高活性的E. coli转座酶突变体(Tn5)与蛋白A/G的融合产物，可与来自多种物种宿主的靶抗体兼容。它也没有表位标签，使其适合于标签介导的CUT&Tag(如FLAG、HA、TY1、V5等)。该产品经过高度纯化，已去除污染的E. coli DNA，并在细胞数量少的情况下进行复杂分析。为了每次实验的标准化以及抗体验证和反应监测，推荐使用SNAP-CUTANA™ nucleosome spike-ins(如EpiCypher 19-1002)。Tn5带有mosaic 接头，可在CUT&Tag中使用。
Adapters
Tn5ME-A: 5’-TCGTCGGCAGCGTCAGATGTGTATAAGAGACAG-3’
Tn5ME-B: 5’-GTCTCGTGGGCTCGGAGATGTGTATAAGAGACAG-3’
Tn5ME-rev: 5’-[phos]CTGTCTCTTATACACATCT-3’
组分
50 mM HEPES-KOH pH 7.2, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% Triton X-100, 50% glycerol.
保存条件
Stable for one year at -20℃ from date of receipt. The protein is not subject to freeze/thaw under these conditions.
数据示例
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FIGURE 1: Protein gel data. CUTANA pAG-Tn5 for CUT&Tag (1 µg) was resolved via SDS-PAGE and stained with Coomassie blue. The migration and molecular weight of the protein standards are indicated. Uncharged pAG-Tn5 monomer is 78.5 kDa, however once charged with DNA, Tn5 dimerizes to a final complex weight of 191 kDa. |
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FIGURE 2: Size distribution of released chromatin. CUT&Tag was performed as described above. Recovered DNA was directly PCR amplified to produce sequenceready libraries. Agilent TapeStation traces for libraries derived from negative control IgG (top) and H3K27me3 (bottom) antibodies are shown. Excised DNA is highly enriched for mononucleosomes (peak at ~300 bp reflects ~150 bp insert size). |
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FIGURE 3: CUT&Tag data. CUT&Tag was performed as described above. Representative sequencing tracks obtained using CUTANA pAG-Tn5 show a 229 kb close up view of the LAMC3 gene. CUTANA pAG-Tn5 produced clear peaks with genomic distribution profiles consistent with the known biological functions of H3K4me3 and H3K27me3 as well as minimal background in the IgG negative control. |
订购详情
货号 | 产品名称 | 规格 |
15-1017 | CUTANA™ pAG-Tn5 for CUT&Tag | 50 Reactions |
15-1117 | CUTANA™ pAG-Tn5 for CUT&Tag | 250 Reactions |
如需了解更多详细信息或相关产品，请联系EpiCypher中国授权代理商-欣博盛生物
