Life-iLab/李记生物 品牌
生产厂家厂商性质
上海市所在地
产品描述
4T1(中文名称:小鼠乳腺癌细胞),来源于小鼠乳腺腺癌,正常的细胞形态为上皮样,贴壁生长。
订购信息
产品名称 | 货号 | 规格 |
4T1 | AX31L184 | T25/1 mL |
运输方式
常温/干冰运输。
使用方法
培养方法
培养基 | RPMI-1640 90%(Gibco: C11875500BT), Fetal Bovine Serum(GIBCO:10099-141)或和元李记特级胎牛血清 (Foetal Bovine Serum)(货号:AC03L055) 10% |
培养条件 | 37℃ 5% CO2 |
消化条件 | 0.25% (w/v) Trypsin-0.53mM EDTA溶液(GIBCO: 25200-056) 或 和元李记0.25%胰蛋的白酶消化液(含EDTA,不含酚红)(货号:AC15L821),消化1-3min |
传代比例 | 1:3-1:5 |
换液时间 | 2-3天换液一次 |
冻存方法
冻存液 | 55%培养基,40%FBS, 5% (v/v) DMSO或 和元李记快速细胞冻存液(无血清、无蛋白)(货号:AC05L033) |
冻存密度 | 1-3 ×106 /管,液氮保存 |
具体操作方法见细胞使用说明书
注意事项
本产品仅限于科学实验研究使用,不得用于临床诊断、治疗等领域。
当收到细胞后,请仔细阅读细胞使用说明书,按规定操作并记录细胞状态(拍照)。我司为客户提供一周内的售后保障,特定情况下可免费重发细胞。
参考文献
Pulaski BA, et al. Immunotherapy with vaccines combining MHC class II/CD80+ tumor cells with interleukin-12 reduces established metastatic disease and stimulates immune effectors and monokine induced by interferon gamma. Cancer Immunol. Immunother. 49: 34-45, 2000. PubMed: 10782864
Pulaski BA, Ostrand-Rosenberg S. Reduction of established spontaneous mammary carcinoma metastases following immunotherapy with major histocompatibility complex class II and B7.1 cell-based tumor vaccines. Cancer Res. 58: 1486-1493, 1998. PubMed: 9537252
Pulaski BA, et al. Cooperativity of Staphylococcal aureus enterotoxin B superantigen, major histocompatibility complex class II, and CD80 for immunotherapy of advanced spontaneous metastases in a clinically relevant postoperative mouse breast cancer model. Cancer Res. 60: 2710-2715, 2000. PubMed: 10825145
Aslakson CJ, Miller FR. Selective events in the metastatic process defined by analysis of the sequential dissemination of subpopulations of a mouse mammary tumor. Cancer Res. 52: 1399-1405, 1992. PubMed: 1540948
相关产品推荐
特级胎牛血清 (Foetal Bovine Serum)(货号:AC03L055)
RPMI-1640 medium (含L-谷氨的酰胺)(货号:AC01L064)
本产品仅限于科学实验研究使用,不得用于临床诊断、治疗等领域。