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沙眼衣原体核酸PCR检测试剂盒

沙眼衣原体核酸PCR检测试剂盒

沙眼衣原体核酸PCR检测试剂盒（PCR检测法）

沙眼衣原体核酸PCR检测试剂盒（PCR检测法）

沙眼衣原体Chlamydien核酸检测试剂盒

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【公司名称】 广州健仑生物科技有限公司
【】    杨永汉 
【】 
【腾讯 】 2042552662
【公司地址】 广州清华科技园创新基地番禺石楼镇创启路63号二期2幢101-3室
【企业文化】

因与肺吸虫及中华支睾吸虫有交叉反应，又因尾蚴供应困难，故不易推广，仅用于流行病学调查及筛选新感染者。3．环卵沉淀试验 取活卵悬液一滴于无菌玻片上，加病人血清等量，加盖玻片石腊密封，置37℃温孵24～48小时，于低倍镜下观察，可见虫卵周围出现球状、指状、丝状、菊花状等形态的沉淀物。观察100个成熟虫卵，计算沉淀物大于10微米的虫卵数所占的百分数，环沉率5%以上者为阳性。感染后7～12天出现反应，阳性率达95%以上，具有早期诊断价值。4．间接血凝试验 采用血吸虫卵抗原致敏红细胞测定病人血清中的抗体，明显凝集者为阳性，特异性与敏感性高，阳性率在90%以上，观察结果快，用血量少及操作简便，本试验与肺吸虫有交叉反应。5．酶联免疫吸附试验（ELISA） 以纯化成虫或虫卵抗原与过氧化物酶或碱性磷酸酶结合，测定病人血清或尿中的血吸虫抗体，敏感性及特异性高，阳性率在95%以上，操作简便，适用于大规模现场使用。6．酶联免疫电泳试验 过氧化物酶标记纯化虫卵抗原后，作对流免疫电泳，检测血清特异性抗体，1/2～1小时出报告，阳性率80%以上。7．循环抗原测定 循环抗原是存在于病人血与尿中的特异性抗原物质，循环抗原阳性，说明宿主体内存在有活的血吸虫感染，具有考核药物疗效的价值。5．肠镜检查及肠粘膜活组织检查 疑似血吸虫的而反复大便检查虫卵性者适用肠镜检查。可见肠粘膜有黄斑、息肉、瘢痕、肥厚、充血、水肿、溃汤等改变。自虫卵堆积处（黄斑及粘膜增厚处）取米粒大小的粘膜组织压片，在显微镜下检查，可查到成堆的虫卵，阳性率在90%以上；在直肠粘膜活检中所见虫卵多成黑色死卵与空卵壳，活卵较少。为此，仅凭活检出卵对近期变性虫不易区别，又因死卵于治疗后可长期留在肠壁中，久不消失，故认为对疗效考核只能作参考。活检时应充分止血，轻取组织、切忌撕拉，以防止出血与穿孔。1．血象 急性期白细胞总数及嗜酸性粒细胞显著增多，白细胞总数多在10～30×109/L，嗜酸性细胞常占20～40%或更高，有时可达80%以上。部分病人嗜酸性粒细胞增高不显著。慢性期嗜酸性粒细胞轻度增多。晚期因脾功能进，白细胞明显减少，并伴有贫血及血小板减少。2．肝功能试验 急性期多因虫卵激导致网状内皮细胞增生

Due to cross-reaction with Pneumocystis and Clonorchis sinensis, it is difficult to popularize because of the difficulty in supplying tail lice. It is only used for epidemiological investigation and screening for new infections. 3. Cyclic egg sedimentation test to take a drop of live egg suspension on a sterile glass slide, add the patient's serum equal amount, cover glass slides, sealed with paraffin, and incubate at 37°C for 24 to 48 hours. Observe under low power microscope, visible around the eggs There are spherical, finger-like, filamentous, chrysanthemum-like precipitates. Observe 100 mature eggs, and calculate the percentage of eggs with sediments larger than 10 μm, and the positive rate is 5% or more. After 7 to 12 days of infection, a positive reaction rate of more than 95% appeared to be of early diagnostic value. 4. Indirect hemagglutination test using schistosomiasis antigen-sensitized red blood cells to determine the antibody in the patient's serum, obvious agglutination was positive, high specificity and sensitivity, the positive rate of more than 90%, observation results fast, with less blood and easy to operate, This test cross-reacted with paragonimiasis. 5. Enzyme-Linked Immunosorbent Assay (ELISA) The detection of schistosomiasis antibodies in serum or urine of patients with purified adult or egg antigen combined with peroxidase or alkaline phosphatase has high sensitivity and specificity, and the positive rate is above 95%. Easy to operate, suitable for large-scale field use. 6. Enzyme-linked immunoelectrophoresis assay After peroxidase-labeled purification of egg antigens, counter-current immunoelectrophoresis was performed to detect serum-specific antibodies. Reports were made 1/2 to 1 hour, and the positive rate was over 80%. 7. Cyclic antigen determination Cyclic antigen is a specific antigen present in the patient's blood and urine, circulating antigen positive, indicating that there is a live schistosome infection in the host body, which has the value of assessing drug efficacy. 5. Colonoscopy and intestinal mucosa biopsy Suspected schistosomiasis and repeated bowel examination of eggs for colonoscopy. Visible intestinal mucosa with macular, polyps, scars, hypertrophy, congestion, edema, ulceration and other changes. Accumulation of mucosal-sized mucous membranes from the site where the eggs were deposited (macular and mucous membrane thickening). Examination under the microscope can reveal piles of eggs, with a positive rate of more than 90%; seen in rectal mucosa biopsies. Eggs are mostly black dead eggs and empty eggshells, with few live eggs. For this reason, it is not easy to differentiate the newly-depopulated worms by the biopsy of eggs, and because the dead eggs can remain in the intestinal wall for a long period of time after treatment, it will not disappear for a long time. Therefore, it is considered that the evaluation of the efficacy can only be used as a reference. The biopsy should be fully stopped bleeding, gentle tissue, avoid tearing to prevent bleeding and perforation. 1. The total number of white blood cells and eosinophils in the acute phase of blood is significantly increased. The total number of leukocytes is usually 10 to 30×10 9 /L. Eosinophils often account for 20 to 40% or more, and sometimes reach 80% or more. Elevation of eosinophils was not significant in some patients. Chronic mild eosinophils increased. Due to the advance of spleen function in the late stage, the white blood cells are significantly reduced, accompanied by anemia and thrombocytopenia. 2. Liver function test: In the acute phase, multiple worm eggs stimulate reticuloendothelial cell proliferation.